摘要
以抗TCRγδ抗体选择性活化扩增γδTIL的方法,可以从小量肿瘤组织,有效扩增其中微量存在的γδTIL。实验证实:本法可在3~4wk内获得较高纯度的γδTIL,纯度可达85%以上。所得γδTIL具有较为完整的受体库(repertoire)。此法获得的γδTIL可用于进一步的功能研究、γδT细胞克隆及过继免疫治疗研究。
γδ T cell invokes interests of many immunologists for its unique nature of antigen recognition and cytotoxic activity in recent years.Tumor infiltrating lymphocyte(TIL) is broadly used in the clinical researches of adoptive immunotherapy because of its strong tumor killing activity,which is 100 times higher than that of lymphokine activated killer cells.This paper reported a method to expand selectively the γδ TIL by using immobilized anti TCRγδ monoclonal antibody.γδ TIL could be effectively expanded from small samples in 3~4 weeks with a purity higher than 85%.The γδ TIL obtained via this method possess a whole repertoire and could be applied in further investigations of functions,adoptive immunotherapy and γδ T cell cloning.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1997年第4期260-262,共3页
Immunological Journal
基金
国家自然科学基金
95年度中国医学科学院科学研究基金
关键词
ΓΔT细胞
肿瘤浸润淋巴细胞
扩增
抗体固相法
γδ T cell,Tumor infiltrating lymphocyte,Expansion,Antibody immobilization