活血化淤药物764-3对胶原生物合成影响的研究

The effect of Huoxue Huayu drug 764 3 on synthesis of collagen

目的：探讨活血化淤药物７６４３对细胞外间质合成的影响。方法：羟脯氨酸法、间接酶联免疫吸附法（ＥＬＩＳＡ）测定博莱霉素Ａ６（ＢＬＭＡ６）诱发的小鼠、大鼠肺纤维化模型肺组织胶原含量及合成，阿利新蓝法测定其糖胺多糖（ＧＡＧ）含量；３ＨＰｒｏ掺入法测定体外培养兔角膜瘢痕成纤维细胞中胶原合成，ＥＬＩＳＡ测定其纤维粘连蛋白（Ｆｎ）含量。结果：７６４３对模型肺组织胶原合成有明显的抑制作用，抑制率５０．５％，尤其能使肺组织中Ⅰ型胶原及ＧＡＧ明显减少；体外实验证实，７６４３可抑制３ＨＰｒｏ及３ＨＴｄＲ掺入成纤维细胞，且使其表面Ｆｎ明显减少。结论：７６４３抗纤维化作用机制的研究，应以细胞外间质组分———胶原合成为基点深入进行。

OBJECTIVE: To study the effect of Huoxue Huayu drug 764 3 on extracellular matrix (ECM). METHODS: Hydroproline determination and ELISA were used for measuring the content of collagen, Alcian blue assay for BLM-A6 induced glycosaminoglycan (GAG) in the lung tissues (rats, mice). Incorporation of 3 H Pro was used for determining the synthesis of collagen, ELISA for the fibronectin (Fn) in cultivated fibroblasts from the rabbit corneal stroma. RESULTS: 764 3 showed inhibition of collagen synthesis in the lung tissues of the animal model, and the inhibition rate was 50.5%. 764 3 as an active component of Huoxue Huayu drug resulted in apparent decrease of contents of collagen type Ⅰ and GAG. In cultivated fibroblasts, both the incorporation of 3 H Pro and the Fn content on surface of cells were significantly inhibited. CONCLUSION: Further research on anti fibrostic mechanism of Huoxue Huayu drug 764 3 should be taken on the basis of collagen biosynthesis.