栀子不同饮片环烯醚萜苷类成分比较研究

Comparative studies on contents of iridoid in processed products from Gardenia jasminoides

目的:对栀子不同饮片环烯醚萜苷类成分进行比较研究。方法:HPLC同时测定2个主要成分(京尼平龙胆二糖苷,京尼平苷)含量,色谱条件为Kromasil C18柱,流动相乙腈-0.3%甲酸水(12∶88),检测波长238 nm,流速1.0 mL.min-1,柱温35℃。结果:2个成分在测定范围内线性关系较好(r>0.999 4),平均回收率分别为101.8%,99.1%。栀子不同饮片中2种环烯醚萜苷类成分含量有差异。结论:所建立的方法准确、可靠,栀子不同饮片的含量变化呈现一定的规律性。

Objective： To compare the contents of iridoid in the different processed products from Gardenia jasminoides. Method： Contents of geniposide and genipin gentiobioside in products were determined simultaneously by HPLC. A kromasil C18 column at 35 ℃ was used with the acetonitrile-0. 3% formic acid anhydrous （ 12： 88） as the mobile phrase. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL·min^-1. Result： The obtained linearity of the two components was good with that the relative coefficients （r） were over 0. 999 4 and the average recoveries were 101.8%, 99. 1%, respectively. Conclusion： The method is simple, accurate with good reproducibility. The results showed contents of two components are difference in the different processed products.