大孔树脂同步分离纯化罗布麻叶总黄酮和总鞣质的研究

Separation and purification process of total flavones and total tannins from Apocynum venetum leaves with macroreticular resin

目的:研究大孔吸附树脂同步分离纯化罗布麻叶总黄酮和总鞣质的工艺条件及参数。方法:以静态饱和吸附量、动态比吸附量、比洗脱量为考察指标,比较不同大孔树脂富集纯化罗布麻叶总黄酮和总鞣质的性能,并对最佳树脂纯化工艺进行筛选。结果:HPD-400型树脂综合性能最好,其吸附分离工艺条件为:上样液以1 BV.h-1吸附流速上样,纯化水冲洗2 BV,60%乙醇洗脱3 BV。结论:经HPD-400大孔树脂纯化后的罗布麻叶有效部位中,总黄酮加总鞣质的质量分数达80%,说明该纯化工艺是可行的。

Objective： To investigate the purification process of total flavones and total tannins from Apocynum venetum leaves with macroreticular resin. Method： The static capacity absorption, dynamic absorption ratio and dynamic elution ratio of different types of macroreticular resins were studied and compared in order to find the best one among the eight macroreticular resins, and the technical process of the type of HPD -400 type macroreticular resin was studied in detail. Result： The type of HPD -400 type macroreticular resin showed the best comprehensive absorption property with the following technological conditions, the current velocity was 1 BV ·h ^-1 , the volume of distilled water was 2 BV, the eluting reagent was 60% ethanol, and the volume of 60% ethanol was 3 BV. Conclusion ： The purity of total flavones and total tannins in the product is up to 80% after purified with HPD-400 macroreticular resin. Therefore, this purification process is feasible.