清心开窍方对AD小鼠学习记忆能力及海马CA1区神经细胞形态的影响

Effect of Qingxin Kaiqiao fang on learning and memory ability and morphology of hippocampal nerve cells in AD mice

目的:探讨清心开窍方对AD模型小鼠学习记忆能力及海马CA1区神经细胞形态的影响。方法:将150只小鼠随机分为5组,即空白组、模型组、清心开窍方高、低剂量组(14.82,7.41 g.kg-1)、脑复康组(0.42 g.kg-1)。除空白组外,其余各组均每日灌服三氯化铝(AlCl3)(200 mg.kg-1)造模,用药各组自造模第7天起,每日上午给予AlCl3灌胃,下午给予相应的药物,每日1次,连续8周,9周后评定疗效,测小鼠学习记忆功能和脑内一氧化氮(NO)、一氧化氮合酶(NOS)及乙酰胆碱脂酶(AchE),及对海马CA1区神经细胞形态学观察。结果:中药各组能明显改善AD小鼠学习记忆能力,与对照组比较有显著性差异(P<0.05,P<0.01);清心开窍方各组能明显减少海马神经细胞的破坏,降低AD小鼠脑组织中NO,NOS含量及AchE活性,与模型组比较有统计学意义(P<0.05,P<0.01)。结论:清心开窍方能改善AD小鼠学习记忆的能力,对海马神经细胞具有保护作用。

Objective： To study the effect of the Qingxin Kaiqiao fang on learning and memory ability and shape hippocampal nerve cells in Alzheimer disease （AD）. Method： One handred and fifty mice were divid into five groups： blank group, model group, two groups of treatment by Qingxin Kaiqiao fang （14. 82, 7. 41 g·kg^-1）, piracetan comparison group （0. 42 g·kg^-1 ）. The model group was orally given A1C13 （200 mg· kg^-1 ） every day. For Qingxin Kaiqiaofang and piracetan groups, AlCl3 treatment was given for 6 days at the beginning, followed by giving orally AlCl3 in the morning and drug in the afternoon for 8 weeks. Then, learning and memory aility, the contents of nitric oxide （ NO）, NO synthase （NOS） and acetylcholinesterase （AchE） in brain, and morphology of hippocampal nerve cells were investigated. Result： Learning and memory ability of Qingxin Kaiqiaofang groups was improved, compared with comparison group; the difference was significant （P 〈 0. 05, P 〈 0. 01 ）. The drug could prevent hippocampal nerve cells from damaged obviously. The contents of NO, NOS and AchE in mice of treatment groups were lower than those of comparison group; the difference was significant （P 〈0. 05, P 〈 0. 01 ）. Conclusion： Qingxin Kaiqiaofang can improve learning and memory ability of AD mice, prote chippocampal nerve cells, and treat Alzheimer disease.