天山雪莲细胞培养物与原药材中紫丁香苷含量的比较

Comparison of Syringin Content Between Cell Cultures and Botanical Drug of Saussurea involucrata

目的比较天山雪莲细胞培养物与原药材中紫丁香苷含量的差异。方法建立了一种HPLC-DAD方法测定紫丁香苷的含量:色谱柱采用Agilent Zorbax SB-C18柱(4·6mm×250mm,5μm),柱温为25℃,流动相为乙腈-水(10:90),流速1·0mL·min-1,检测波长220nm。结果与天山雪莲原药材相比,细胞培养物中紫丁香苷的含量在液体培养和固体培养方式下,分别提高了20·96和55·50倍。结论利用细胞培养方式生产紫丁香苷具有良好的开发前景。

OBJECTIVE To compare the syringin content in cell cultures with that in botanical drug of Saussurea involucrata. METHODS A HPLC-DAD method was developed as follows： chromatographic separation was achieved on an Agilent Zorbax SB - C18 column （4. 6 mm ×250 mm,5 μm） at 25 ℃ and the mobile phase was a mixture of acetonitrile-water （10：90） detected at 220 nm at a flow rate of 1.0 mL · min^-1. RESULTS Compared with the botanical drug, the content of syringin in cells was improved 20. 96 times by liquid culture and 55. 50 times by solid culture. CONCLUSION It has a good prospect to acquire syringin by plant cell culture.