洋参二醇皂苷注射液对犬实验性心肌梗死的保护作用及其机制

Protective Effect of Panax quinquefolium Diolsaponins for Injection and Its Mechanism on Experimental Myocardial Infarction in Dogs

目的研究洋参二醇皂苷注射液(IPQDS)对犬实验性心肌梗死的保护作用及其机制。方法通过麻醉犬结扎左冠状动脉前降支6h制备急性心肌梗死模型,应用IPQDS进行治疗,观测24个点心外膜心电图,计算心肌缺血程度(△Σ-ST)、缺血范围(△N-ST)及心肌梗死面积(MIS),测定血清磷酸肌酸激酶(CPK)、天门冬氨酸氨基转换酶(AST)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、脂质过氧化物(LPO)、游离脂肪酸(FFA)含量及全血黏度及血浆黏度。结果IPQDS能使实验性心肌梗死犬的△Σ-ST及△N-ST明显降低,MIS明显缩小;明显降低血清CPK、LDH及AST活性;降低血清LPO及FFA含量;降低全血低、中、高切黏度及血浆黏度,明显提高SOD及GSH-Px活性。结论IPQDS对犬实验性心肌梗死具有明显保护作用,作用机制可能与改善心肌代谢,降低血液黏度,提高内源性抗氧化酶活性及减轻氧自由基的损伤有关。

OBJECTIVE To study the protective effect ofPanax quinquefolium Diolsaponins the lnjection（IPQDS） and its mechanism on experimental myocardial infarction in dogs. METHODS The acute myocardial infarct model was prepared by left anterior descending coronary occulusion for 6 h in anesthetized dogs. After treatment by IPQDS,the changes of myocardial ischemic extent （ Δ∑- ST）,myocardial ischemie area （Δ N-ST）, myocardial infarct size （MIS）, the activities of creatine phosphokinase （CPK）, aspartate aminotransferase （ AST）, lactate dehydrogenase （ LDH）, superoxide dismutase （SOD） and glutathione peroxidase （ GSH-Px）, the contents of lipid peroxidation （LPO） and free fatty acid （FFA） in serum were determined. At the same time,the viscosity of blood were significantly reduced,the activities of CPK,AST and LDH in serum,the contents of LPO and FFA in serum,and the viscosity of blood and plasma were declined,while the activities of SOD and GSH-Px in serum were increased. CONCLUSION IPQDS have protective effect on experimental myocardial infarction in dogs ,which may be related to improving myocardial metabolism, decreasing the viscosity of blood and plasma,increasing the activity of antioxidiase,and eliminating the free radicals etc.