益气明目口服液对缺血再灌注损伤模型大鼠的疗效观察

Effects of Yiqimingmu solution on models of ischemia reperfusion injury in rats retina

目的:探讨益气明目口服液对实验性高眼压所致视网膜缺血再灌注损伤模型大鼠的治疗作用。方法:SD大鼠60只,随机分为正常对照组、缺血再灌注损伤模型组、益气明目口服液组。应用前房恒压灌注液体升高眼内压的方法建立大鼠缺血再灌注损伤模型.造模前分别采用等量纯化水和益气明目口服液灌胃治疗7 d。缺血再灌注损伤后7 d,观察视网膜电图的改变,检测视网膜组织中超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量,并进行视网膜透视电子显微镜和光学显微镜的组织学观察。结果:缺血再灌注损伤后7 d,益气明目口服液对实验大鼠缺血再灌注损伤后ERG a波及b波振幅的降低均有保护作用.益气明目口服液可以抑制缺血再灌注损伤后视网膜中SOD活性的降低及MDA含量的升高;通过视网膜透视电子显微镜和光学显微镜的组织学观察,益气明目口服液组大鼠视网膜的病理组织学损害较缺血再灌注损伤模型组明显减轻。结论:益气明目口服液对视网膜缺血再灌注损伤有保护作用,是一种治疗视网膜缺血性疾病的有效中药复方制剂。

OBJECTIVE To investigate the experimental therapeutic effects of traditionou chinese Medicine Yiqimingmu solution on rats models with retinal ischemia reperfusion（RIR） injury induced by high intraocular pressure. METtlODS Sixty SD rats were randomly divided into： normal control group, retinal ischemia reperfusion injury model group, Yiqimingmu solution group. High intraocular pressure was induced by anterior chambers infusing with liquid to mate RIR injury model. Distilled water and Yiqimingmu solution were respectively given orally to the rats for 7 days before ischemia reperfusion injury. On the 7th day after RIR injury, the change of electroretinogram was observed, the content of malondialdehyde（MDA） and activity of superoxide dismutase（SOD） in rats retina were measured, and the histopathologic changes were observed with transmission electron microscopy and light microscopy. RESULTS On the 7th day after RIR injury, Yiqimingmu solution had protective effect on fall of ERG a wave and b wave amplitude induced by RIR injury. The activity of SOD in Yiqimingmu solution group was higher than in RIR model group（P〈0. 01）, the content of MDA in Yiqimingmu solution group was lower than in RIR injury model group（P〈0. 01）. The histopathologic injury of rats retina in Yiqimingmu solution group was slighter than in RIR injury model group by means of transmission electron microscopy and light microscopy. CONCLUSION Yiqimingmu solution has protective effect on rats retina ischemia reperfusion injury, and is an effective traditional Chinese medicine to treat diseases of retinal ischemia reperfusion injury.