毛果芸香碱对实验性心律失常的保护作用

Pharmacological effect of pilocarpin on arrhythmia in nimal studies

目的:观察毛果芸香碱对实验性心率失常的影响。方法:分别以乌头碱,氯化钡和哇巴因制备实验性动物心律失常模型。观察毛果芸香碱的干预作用。结果:毛果芸香碱可显著延迟乌头碱引起的大鼠室性心律失常的出现(P<0.05),延长出现心律失常后的存活时间(P<0.05);能明显延迟氯化钡引起的大鼠双相室性心律失常的出现(P<0.01).缩短心律失常的持续时间(P<0.01);能显著延长哇巴因引起豚鼠出现心律失常后的存活时间(P<0.05)。毛果芸香碱的上述作用可被M_3受体阻断剂4-DAMP完全逆转。结论:毛果芸香碱具有对抗乌头碱和氯化钡诱发大鼠,哇巴因诱发豚鼠心律失常的作用,提示其具有良好的抗心律失常作用;毛果芸香碱通过激动大鼠和豚鼠心肌M_3受体而产生抗心律失常的作用。

OBJECTIVE To investigate the antiarrhythmic effects of pilocarpine in rat and guinea pigs. METHODS Aconitine,BaC12 and ouabain were used to induce experimental arrhythmic models in Wistar rats and guinea pigs. Effects of pilocarpine on the occurrence of arrhythmias was observed. RESULTS Pilocarpine substantially depressed the occurrence of arrhythmias induced by aconitine,BaCh and ouabain. In the groups of aconitine, the occurrence of arrhythmias were delayed obviously （P〈0. 05）and the survival time after the occurrence of arrhythmias was prolonged obviously （P〈0. 05）. In the groups of BaC12, the occurrence of arrhythrnias was delayed obviously（P〈0.01） and the persistence of arrhythmias were decreased obviously （P〈0. 01）. In the groups of ouabain, the survival time after the occurrence of arrhythmias was also prolonged obviously （P〈0. 05）. Meanwhile, 4 DAMP（antagonist of M3 receptors）could reverse the effects of pilocarpine completely. CONCLUSION Pilocarpine has antiarrhythmic effects on the arrhythmias induced by aconitine, BaCLa and ouabain. Pilocarpine plays the antiarrhythmic roles by activating cardiac M3 receptors of Wistarrats and guinea pigs.