抗青枯病转多肽抗生素基因烟草的选育

The breeding of transgenic tobacco with peptide antibiotic genes against bacterial wilt

分别于温室和田间条件下,对转单价apidaecin、shiva-I及双价apidaecin+shiva-I基因的14个烟草株系材料的T1和T2后代群体进行青枯病抗性筛选鉴定及农艺性状评价。温室盆栽试验中,于5～6叶龄期采用伤根法进行接种。接种后15天,转apidaecin、shiva-I和apidaecin+shiva-I基因烟草的病情指数较对照分别下降了16.3%、15.2%和59.7%。田间自然病圃筛选鉴定结果表明:B43-5-4、B43-5-1、B43-5-2、B43-5-3和B43-4-1等5个株系材料病情指数较起始品种K326降低21%～70%,且农艺性状与起始品种K326无显著差异。

14 T1 and T2 transgenic tobacco lines with apidaecin, shiva-I and apidaecin ＋ shiva-I genes were evaluated for bacterial wilt resistance and agronomic trait both in greenhouse and field plot, 15 days after inoculation with R. solanacearum under greenhouse conditions. All three kinds of transgenic plants showed enhanced resistance. The transgenic plants harboring apidaecin ＋ shiva-I cassette showed the highest disease resistance among all three groups of transgenic plants. Five T2 transgenic lines （ B43-5-4, B43-5-1, B43-5-2, B43-5-3 and B43-4-1） decreased disease indices by 21% -70% in field trial. In the meantime, they exhibited no significant difference in agronomic traits and quality by comparing with original cuhivar K326.