无机抗菌材料抗病毒效果的实验室检测方法研究

Study on detection of antivirus effective for inorganic antibiotic materials

目的：建立无机抗菌材料抗病毒效果的实验室检测方法。方法：用乙型肝炎表面抗原（HBsAg）破坏试验和脊髓灰质炎病毒灭活试验检测了纳米TiO2和镀银纤维两种无机抗菌材料抗病毒效果。结果：纳米TiO2瓷碟在普通光照（高压汞灯、非直射阳光）下4 h对乙肝病毒（HBsAg）的杀灭率达87.50%-93.75%，纳米TiO2镍网与紫外灯配合2、5、10 min对HBsAg的杀灭率分别为99.80%、99.90%、100%；镀银纤维在日光灯照射下5、10、30、60 min对病毒的杀灭率分别为73.10%、96.84%、99.99%和大于99.99%。结论：无机抗菌材料具有良好的抗病毒功效，建立的抗病毒效果的实验室检测方法是合理、可行的，适用于同类无机抗菌材料抗病毒功效的检测。

Objective： To develop of methods for detection of the antivirus effective for inorganic antibiotic materials in vitro. Methods： Using ELISA to detect the destructive rate of Hepatitis B surface antigen for Nano - TiO2 antibacterial materials, the inhibitive effect to Polio virus for Nano - Silver Base Inorganic Antibacterial fiber was observed. Results： The anti - HBV effect of Nano - TiO2 antibacterial materials was observed by ELISA. The destruct HBsAg rate of nano - TiO2 ceramic plate was 87.50%-93.75% when it was exposed to high Hg lamp irradiation for 4hours, the destructive rates to HBsAg of nano- Ni net were 99. 80% ,99.90% and 100% respectively,under ultraviolet irradiation for 2,5,10 minutes. After being exposed to daylight lamp for 5,10,30 and 60 minutes, the kill rate of Nano - Silver fiber to polio virus was 73. 10% ,96. 84% ,99. 99% and 〉 99. 99% respectively. Conclusion：Inorganic antibiotic mate.rials have good antivirus activity. The methods is feasible and could be used to detect the effect of antivirus for similar materials.