大蒜素对大鼠缺血再灌注肝脏损伤的保护作用

The protective effect of allicin on hepatic ischemia reperfusion injury in rats

目的观察大蒜素对大鼠缺血再灌注肝脏损伤的保护作用。方法雄性SD大鼠,随机分为假手术组、缺血再灌注组(I/R组)和I/R加大蒜素处理组。肝脏缺血再灌注后,分别观察血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)及乳酸脱氢酶(LDH)含量的变化,同时分析肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性。结果肝脏缺血再灌注后,与假手术组比较,血清中ALT、AST、LDH及肝组织中MDA含量均显著增加,而SOD活性显著的降低(P<0.01);经大蒜素处理后,与缺血再灌注组相比较,血清中ALT、AST、LDH及肝组织中MDA含量均降低,而SOD活性增高(P<0.05)。结论大蒜素对大鼠肝脏缺血再灌注损伤有一定的保护作用。

Objective To investigate the protective effect and its role of allicin on hepatic ischemia reperfusion injury in rats. Methods Male Sprague-Dawley rats were randomly divided into three groups： pseudo-operation normal control group, hepatic ischemia reperfusion injury model group, allicin group. After hepatic ischemia and reperfusion, to analyze the level of ALT, AST and LDH in the serum and observe the change of MDA and SOD activity of the hepatic tissue. The injury circumstance of the liver tissue in each group were compared by HE staining. Results After hepatic ischemia reperfusion, the MDA level and the content of ALT, AST and LDH increased, and the SOD activity decreased obviously comparing with the normal group（P〈0.01）, Comparing to the hepatic ischemia reperfusion injury model group, the MDA level and the content of ALT,AST and LDH decreased, and the SOD activity increased（P〈0.05）. Conclusions The allicin have a prominent protective effect on hepatic ischemia reperfusion injury in rats.