温敏性壳聚糖共聚膜的制备与细胞吸附/脱附行为

Preparation of Thermoresponsive Chitosan Copolymer and Its Cytocompatibility and Detachability

将丙烯酸(AAc)与壳聚糖(CS)反应,合成了壳聚糖大单体(CS-AAc),再用CS-AAc与N-异丙基丙烯酰胺(NIPAAm)共聚,制备P(CS-AAc-NIPAAm)共聚物.通过红外光谱和X射线光电子能谱等分析证实了产物的结构和组成.对P(CS-AAc-NIPAAm)共聚膜的动态接触角及对细胞的吸附与脱附行为研究发现,共聚膜表现出良好的温度敏感性,其表面成功地种植了成纤维细胞(L929).当环境温度降低后,共聚膜表面细胞自动脱附,从而避免了使用酶解法脱附细胞造成的细胞功能损伤.

In this study, Chitosan（CS） based macromer was used to modify NIPAAm to make a novel temperature-responsive film for cell adhesion and detachment. CS was coupled with acrylic acid （AAc） to prepare a macromer（CS-AAc）. Then, it was copolymerized with N-isopropylacrylamide （NIPAAm） to synthesize a P（CS-AAc-NIPAAm） film. The novel copolymer obtained was characterized via FTIR and XPS. There was thermo-response for the film against water, and this phenomenon was coincided with the data of dynamic contact angle against water. Moreover, the cytocompatibility of the film was investigated with fibroblast culture in vitro. The result reveal that the film exhibited a good cytocompatibility, when the temperature declined, it had the cell detachability.