大孔吸附树脂纯化山茱萸总皂苷的动态洗脱条件研究

Study on Dynamic Desorption Conditions of Total Saposins from Cornus officinalis by Macroporous Resins

运用单因素试验和正交试验研究了山茱萸总皂苷在HPD-300大孔吸附树脂上的动态洗脱工艺,经极差分析和方差分析确定了其最佳操作条件。结果表明,最佳蒸馏水洗脱条件为:水洗流速1.0BV/h,用水量2.2BV,水洗时间2.2h;最佳洗脱剂洗脱条件为乙醇浓度50%,洗脱流速2.0BV/h,洗脱剂用量4.5BV。

This experiment studied dynamic de-adsorption technology of total saposins from Comus officinalis in HPD-300 macroporous resin by single factor tests and orthogonal test designs. The optimal manipulation conditions were ascertained by variance analyses. The results showed that the optimum desorption conditions are： flowing rate 1.0 BV/h, volume 2.2 BV, and desorption time 2,2 h; The best desorption conditions by alcohol are 50% concentration, flowing rate 2.0 BV/h, and desoprtion volume 4.5 BV.