超声辅助同时提取条斑紫菜多糖及藻胆蛋白工艺的优化

Optimization Technique of Synchronous Ultrasonic-assisted Extraction of Polysaccharide and Phycobiliprotein from Porphyra yezoensis

以条斑紫菜为原料,通过单因素和正交试验,确定了超声辅助同时提取条斑紫菜多糖和藻胆蛋白的最佳工艺条件。结果表明,超声辅助同时提取条斑紫菜多糖和藻胆蛋白的最佳工艺条件为:超声60min,功率880W,水浴30℃,4h,料液比1:50。利用本法优化后的工艺同时提取条斑紫菜多糖和藻胆蛋白,紫菜多糖提取得率为34.03%,藻胆蛋白提取得率为1.2%,藻红蛋白纯度为0.356。

Using Porphyra yezoensis as the raw material, single factor and orthogonal tests were conducted to obtain the optimum conditions of synchronous ultrasonic-assisted extraction of polysaccharide and phycobiliprotein. The results showed that the optimum conditions are ultrasonic power 880 W, ultrasonic time 60 min, water extracting temperature 30~C for 4 hours, and ratio of solid-liquid 1：50. Using the optimum conditions, the extraction percent of polysaccharide and phycobiliprotein is respectively 34.03% and 1.2%, and phycoerythrin purity is 0.356.