“Amano”β-糖苷酶水解大豆异黄酮技术的研究

Study on Hydrolysis of Soybean Isoflavones by β-Glycosidas "eAmano"

研究了"Amano"β-糖苷酶水解大豆异黄酮技术工艺。通过单因素试验对水解过程中的不同影响因素进行了考察。运用正交试验优化了"Amano"β-糖苷酶水解大豆异黄酮的反应条件。该酶水解黄豆苷的最佳条件为:水解时间140min,加酶液浓度33μg/ml,pH4.5,温度50℃;水解染料木苷的最佳条件为:水解时间140min,加酶液浓度33μg/ml,pH4.5,温度60℃。验证实验证实用最佳反应条件水解大豆异黄酮可使黄豆苷的水解率达到98.2%,染料木苷的水解率达到94.2%。

The technique of hydrolysis of soybean isoflavones by β-glycosidase ＂Amano＂ was studied in this study. The different influencing factors during the process of enzyme hydrolysis were studied through single factor tests. The reaction conditions of hydrolysis by β-glycosidase＂Amano＂ were optimized through orthogonal test. The optimal reaction conditions of hydrolysis of daidzin were： time 140 min, the concentration of enzyme 3 3μg/ml, pH 4.5, temperature 50℃. The optimal reaction conditions of hydrolysis of genistin were： time 140 rain, the concentration of enzyme 33μg/ml, pH 4.5, temperature 60℃. The identified test proved the hydrolysis ratio of daidzin attained 98.2% and the hydrolysis ratio of genistin attained 94.2% under the optimal conditions.