采用正交设计法对桑黄菌丝体液体培养基的优化

Optimization of Liquid Culture Medium for Phellinus linteus Mycelia by Orthogonal Test

以玉米渣和葡萄糖为复合碳源,以麸皮和蛋白胨及酵母粉为复合氮源,采用L9(34)正交设计法优化桑黄菌丝体液体培养基,并为此初步确定了液体培养条件。实验结果表明:最佳培养基为玉米渣3%、麸皮3%、葡萄糖3%,KH2PO40.3%、MgSO4·7H2O0.15%、VB120μg/100ml、VB230μg/100ml。在32.5℃、pH自然、摇床转速130r/min、摇床培养192h的条件下,其菌丝体生物量平均得率为4.4g/100ml。

Corn flour as mixed carbonic source and bran ＋ peptone ＋ yeast as mixed nitrogenous source, the liquid culture medium of Phellinus linteus mycelia was optimized by orthogonal test in this study. The results showed that the optimal culture medium comprised corn flour 3%, bran 3%, glucose 3%, KH2PO4 0.3%, MgSO4·7H2O 0.15%, VB1 20μg/100 ml, VB2 30μ g/100 ml, and on the culture conditions, of culture temperature 32.5℃, natural pH, rotation speed 130 r/min and culture cycle 192 h, the average mycelial biomass was 4.4 g/100 ml.