摘要
目的:通过研究葛根总黄酮对运动大鼠骨四头肌转铁蛋白受体(transferrin receptor,TfR)基因表达的影响,分析葛根总黄酮抗疲劳的分子生物学机制。方法:将SD大鼠随机分为三组:安静对照组(A)、运动组(B)和服药组(C)。B组和C组进行7周的大强度耐力跑台训练,随后抽提各组大鼠骨四头肌TfR mRNA,与Cy3和Cy5标记的cDNA混合探针杂交,通过芯片扫描仪对其荧光强度进行扫描,并用计算机对结果进行分析。结果:基因表达谱显示,与安静组比较,运动组大鼠骨四头肌TfR基因表达下调;与运动组比较,服药组大鼠骨四头肌TfR基因表达显著上调。结论:葛根总黄酮对运动大鼠骨四头肌TfR基因表达有明显上调作用,而TfR基因表达上升与运动中铁的转运密切相关,可作为易感基因在疲劳的分子生物学机制和抗疲劳功能食品筛选中进一步研究。
Objective： By genechip cDNA microarray, the biomolecular mechanism of anti-fatigue of Pueraria flavonid was studied in the quadriceps of the exercise-training rats. Methods： SD rats were randomly divided into three groups： normal rest group（A）, exercise-training group（B） and administered-drug group（C）. B group and C group rats were trained with high intensity 7 weeks by treadmill. The total RNAs were isolated from the quadriceps tissue, and were purified to mRNAs by oligotex. The purified mRNA were reversely transcribed to cDNA with hybridization probes labeled by fluorescent-dUTP. The genechip was scanned for the fluorescent signals and showed differences by the computer analysis. Results： The TfR gene was down-regulated expression in the quadriceps of exercise-training rats compared with of rest rats and obviously up-regulated expression in the quadriceps of administered-drug rats compared with of exercise-training rat. Conclusion： TfR gene expression in quadriceps of the waining rats is regulated by Pueraria flavonid, and its expression has an effect on fatigue and plays a role in the study of fatigue mechanism and screening anti-fatigue functional food as a sensitive gene.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第5期412-415,共4页
Food Science
