灵芝肽对小鼠半乳糖胺致肝损伤的保护作用

Hepatoprotective Effects of Ganoderma lucidum Peptides Against D-galactosamine-induced Liver Injury in Mice

目的:研究灵芝肽(GLP)对D-半乳糖胺(D-Gal)诱导的小鼠化学性肝损伤的保护作用。方法:将小鼠随机分成正常组、模型组、GLP低、中、高剂量组,每天灌胃一次,GLP组分别灌胃GLP60、120、180mg/kgbw,正常组、模型组灌胃等量生理盐水,2周后模型组和GLP组腹腔内注射D-Gal(750mg/kgbw),诱导小鼠肝损伤。通过试剂盒的方法分别检测各组小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活力,肝组织中丙二醛(MDA)、谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力;并进行肝组织病理学镜检。结果:与模型组比,以180mg/kgbw给予GLP,可显著降低小鼠血清ALT、AST的活性和肝组织中MDA含量,提高肝组织中SOD的活力与GSH的含量(p<0.05～0.01),肝组织病理学镜检结果显示已接近正常组。结论:GLP对D-Gal诱导的小鼠肝损伤有很好的保护作用。

Objective： To research the hepatoprotective effects of Ganoderma lucidum peptides （GLP） against D-galactosamine （D-Gal）induced liver damage in mice. Method： Mice were randomly divided into five groups, namely normal, model and three GLP-treated groups. GLP-treated groups were daily given （ig） the doses at 60, 120 and 180 mg GLP/kg bw respectively. Normal and model groups simultaneously received the same amount of physiological saline for 2 weeks. Then the mice from model group and GLP-treated groups were treated with 750 mg/kg bw D-Gal （ip）. Aspartate transaminase/alanine transaminase （AST/ALT） activities in serum, malondialdehyde/glutathione （MDAJGSH） levels and superoxidedismutase （SOD） activity in liver were measured by the detection kits according to the manufacturer＇s protocols. The liver histopathological characters were observed under light microscopy. Results： At the dose of 180mg/kg bw GLP, the ALT/AST activities in serum and MDA lever in liver were significantly reduced, SOD activity and GSH content in liver were significantly increased （p 〈 0.05～0.01） when compared to model group. The liver histopathological characters were close to normal groups＇. Conclusion： GLP possesses potent hepatoprotective effects against D-Gal-induced liver damage in mice.