摘要
目的:研究灵芝肽(GLP)对D-半乳糖胺(D-Gal)诱导的小鼠化学性肝损伤的保护作用。方法:将小鼠随机分成正常组、模型组、GLP低、中、高剂量组,每天灌胃一次,GLP组分别灌胃GLP60、120、180mg/kgbw,正常组、模型组灌胃等量生理盐水,2周后模型组和GLP组腹腔内注射D-Gal(750mg/kgbw),诱导小鼠肝损伤。通过试剂盒的方法分别检测各组小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活力,肝组织中丙二醛(MDA)、谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力;并进行肝组织病理学镜检。结果:与模型组比,以180mg/kgbw给予GLP,可显著降低小鼠血清ALT、AST的活性和肝组织中MDA含量,提高肝组织中SOD的活力与GSH的含量(p<0.05~0.01),肝组织病理学镜检结果显示已接近正常组。结论:GLP对D-Gal诱导的小鼠肝损伤有很好的保护作用。
Objective: To research the hepatoprotective effects of Ganoderma lucidum peptides (GLP) against D-galactosamine (D-Gal)induced liver damage in mice. Method: Mice were randomly divided into five groups, namely normal, model and three GLP-treated groups. GLP-treated groups were daily given (ig) the doses at 60, 120 and 180 mg GLP/kg bw respectively. Normal and model groups simultaneously received the same amount of physiological saline for 2 weeks. Then the mice from model group and GLP-treated groups were treated with 750 mg/kg bw D-Gal (ip). Aspartate transaminase/alanine transaminase (AST/ALT) activities in serum, malondialdehyde/glutathione (MDAJGSH) levels and superoxidedismutase (SOD) activity in liver were measured by the detection kits according to the manufacturer's protocols. The liver histopathological characters were observed under light microscopy. Results: At the dose of 180mg/kg bw GLP, the ALT/AST activities in serum and MDA lever in liver were significantly reduced, SOD activity and GSH content in liver were significantly increased (p 〈 0.05~0.01) when compared to model group. The liver histopathological characters were close to normal groups'. Conclusion: GLP possesses potent hepatoprotective effects against D-Gal-induced liver damage in mice.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第5期416-419,共4页
Food Science
基金
国家自然科学基金项目(30570190)
国家科技支撑计划项目(2006BAD27B09-4)
关键词
灵芝肽
D-氨基半乳糖
肝损伤
保肝
Ganoderma lucidum peptides (GLP)
liver damage
D-Galactosamine (D-Gal)
hepatoprotective effect