单核细胞趋化蛋白-1基因多态性与冠状动脉介入治疗后再狭窄的相关性研究

Association of the monocyte chemoattractant protein-1 -2518A/G gene polymorphism with coronary lesions and in-stent restenosis after percutaneous coronary intervention

目的探讨单核细胞趋化蛋白1（MCP-1）启动子区-2518A／G基因多态性与冠状动脉（冠脉）粥样硬化病变进程及经皮冠脉腔内成形术（PCI）后再狭窄的相关性。方法对276例接受PCI并进行冠脉造影随访的患者，采用PCR—RFLP方法进行MCP-1—2518A／G多态性检测；按冠脉造影结果分为再狭窄组（113例）和无再狭窄组（163例），判定冠脉血管病变及再狭窄与MCP-1—2518A／G多态性的相关性。结果MCP-1—2518A／G基因型频率为：AA纯合子21．0％，GG纯合子34．1％，AG杂合子44．9％，3种基因型血管病变支数和血管平均狭窄程度，差异均无统计学意义（P〉0．05）。再狭窄组中AA、AG和GG基因型频率分别为23．9％、40．7％和35．4％，无再狭窄组分别为19．0％、47．9％和33．1％，差异无统计学意义（P=0．446）。再狭窄组中-2518A和G等位基因频率分别为44．2％和55．8％，无再狭窄组分别为42．9％和57．1％，差异无统计学意义（P=0．761）。结论冠脉粥样硬化进程及PCI术后再狭窄可能与MCP-1—2518A／G基因多态性无相关性。

Objective To investigate the association of monocyte chemoattractant protein-1 （MCP-1） promoter -2518A/G gene polymorphism with coronary lesions and in-stent restenosis in Tianjin Chinese population. Methods Two hundred and seventy six patients who underwent percutaneous coronary intervention （PCI） and coronary angiography during follow-up were enrolled in the study. The MCP-1 gene promoter polymorphism at position -2518 was determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Results The frequencies of three genotypes of MCP-1 -2518A/G polymorphism were 21.0% AA, 34.1% GG, 44.9% AG, respectively. There were no statistical differences in the number and the mean degree of stenosis vessels before PCI among 3 genotype groups （all P）0.05）. 113 cases developed in-stem restenosis and 163 cases were free from restenosis. In restenosis group, the AA, AG and GG genotype frequencies were 23.9%, 40.7%, 35.4%, against 19.0%, 47.9% and 33. 1% in non- restenosis group （P=0.446）. The frequencies of-2518A and G allele were 44.2%, 55.8% in restenosis group versus 42. 9%, 57.1% in non-restenosis group（P=0. 761）. Conclusions The polymorphism of MCP-1-2518 A/G gene may be associated with neither atherosclerosis nor the in-stem restenosis.