白介素-10对大鼠脑缺血再灌注血管内皮细胞间黏附分子-1和P-选择素的抑制作用

Inhibiting effect of interleukin-lO on expressions of intercellular adhesion molecule-1 and P-selectin in cerebral ischemia-reperfusion foci of rats

目的探讨白介素-10（Interleukin-10，IL-10）对大鼠脑缺血再灌注血管内皮细胞表达细胞间黏附分子-1（intercellular adhesion molecule-1，ICAM-1）及P-选择素的抑制作用。方法60只成年雄性Sprague-Darley大鼠随机分为假手术组，脑缺血再灌注组（6h组、24h组、48h组），溶剂对照组（6h组、24h组、48h组）和IL-10干预组（6h组、24h组、48h组），每组各6只。参照Longa法建立大鼠大脑中动脉阻塞（middle cerebral artery occlussion，MCAO）脑缺血再灌注模型，IL-10干预组于造模成功后0．5h经侧脑室注射IL-10（1μg溶于5mol／LNaP 5μl），溶剂对照组经侧脑室注射溶剂（5mol／LNaP 5μl）。免疫组织化学法和RT-PCR法检测ICAM-1和P-选择素表达的动态变化。结果脑缺血再灌注6h组、24h组、48h组血管内皮细胞表达ICAM-1分别为[（31．60±1．85）、（54．11±2．32）、（29．39±5．16）个]和P-选择素[（48．61±2．30）、（50．44±4．38）、（37．89±4．74）个]，较假手术组[（7．61±1．14）个]和[0个]显著上调（P〈0．01）；IL-10干预组ICAM-1[（24．06±2．42）、（42．00±3．26）、（45．28±2．91）个]和P-选择素表达[（27．28±1．84）、（42．78±2．51）、（36．00±3．22）个]，较溶剂对照组显著下调（P〈0．05）。结论IL-10可抑制大鼠脑缺血再灌注血管内皮细胞ICAM-1及P-选择素的表达。

Objective To investigate the inhibiting effect of interleukin-10 on the expressions of intercellular adhesion molecule-1（ICAM-1） and P-selectin in cerebral i schemia and reperfusion loci （I/ R） in rats. Methods Sixty adult male Sprague-Dawley rats were assigned to 4 groups randomly： Sham operated group（n=6）, cerebral I/R group （6, 24, 48 hours post I/R, each had 6 rats）, vehicle group （6, 24, 48 hours post I/R, each had 6 rats）and IL-10 treatment group （6, 24, 48 hours post I/ R, each had 6 rats）. I/R rat model was established by middle cerebral artery occlusion（MCAO） with Longa＇s thread method. Immunohistochemical staining and retro-transcriptase polymerase chain reaction were used to detect the expressions of ICAM-1 and P-selectin. Results ICAM-1（31.60± 1.85, 54.11±2.32, 48.61±2.30） and P-selectin （29.39±5.16, 50.44±4.38, 37.89±4.74） expressions on endothelial cells 6, 24 and 48 hours post I/R were significantly upregulated as compared with sham group（7.61±1.14,0, respectively）（all P〈0.01）,and the expressions of ICAM-1 （24.06±2.42, 42.00±3.26, 45.28±2.91） and P-selectin（27.28± 1.84, 42.78±2.51, 36.00± 3.22） on endothelial cells of IL-10 treatment group 6h, 24h, 48 h post I/R were significantly suppressed at any timepoint in comparison with Vehicle group （P〈0.05）. Conclusions IL-10 could inhibit the expressions of ICAM-1 and P-selectin on endothelial cells of cerebral I/R loci in rats.