乙酰肝素酶在膀胱移行细胞癌中的表达及临床意义

Expression of heparanase in the bladder transitional cell carcinoma and its clinical significance

目的:探讨乙酰肝素酶(Heparanase,HPA)在膀胱移行细胞癌中的表达及临床意义。方法:应用逆转录-聚合酶链反应(RT-PCR)及免疫组织化学方法检测68例膀胱移行细胞癌、24例癌旁组织、15例正常膀胱组织中HPA mRNA的水平及蛋白表达定位,并结合患者临床病理指标及预后进行分析。结果:膀胱移行细胞癌中HPA mRNA检出阳性41例(60.3%),24例癌旁组织中阳性1例(4.2%),15例正常膀胱组织中全部阴性,分别与癌组织组比较差异有统计学意义(P<0.01),HPA mRNA的表达在膀胱移行细胞癌不同病理分级、临床分期及淋巴结转移与否分组中显著不同(P<0.01)。免疫组织化学结果显示,HPA定位于细胞质及细胞核,阳性率为77.9%(53例),在膀胱移行细胞癌淋巴结转移与否分组中显著不同(P<0.01)。结论:HPA在膀胱移行细胞癌的生长浸润、侵袭转移中可能发挥重要作用,可为膀胱移行细胞癌生物学行为和预后的判断提供较客观指标。

Objective：To explore the expression of heparanase（HPA） and its correlation with the clinical pathological features and angiogenesis in bladder transitional cell carcinomas Methods： HPA mRNA level was detected with RT-PCR methods and immunohistochemistry staining in 68 bladder cancer and 24 adjacent cancer tissue and 15 normal bladder tissue. Expression of HPA mRNA was analyzed with their relation to the clinical pathological features in bladder cancer. Results：The positive of HPA mRNA was 60. 3%（41/68） ,4.2%（1/24） ,0% （0/15） in 68 bladder cancer and 24 adjacent cancer tissue and 15 normal bladder tissue respectively. The expression of HPA mRNA has much correlation with pathological grades and lymph node metastasis of bladder cancer. The positive of HPA protein was 77.9% （53/68） in bladder cancer. The expression of HPA protein has much correlation with lymph node metastasis. Conclusion： HPA may play an important role in the growth, invasion and metastasis of bladder cancer. The expressions of HPA was an useful marker of the biological behavior of bladder cancer and an independent prognosis factor for the patients with bladder cancer.