PD-L1、PD-L2 mRNA在OLP组织中的表达研究

Study on the expression of PD-L1 and PD-L2 mRNA in oral lichen planus.

目的:研究PD-L1和PD-L2 mRNA在OLP损害组织中的表达。方法:采用Real-timePCR方法,检测PD-L1和PD-L2 mRNA在20例OLP不伴异常增生、10例OLP伴轻度异常增生损害组织中的表达水平,并以10例健康人正常黏膜为对照。结果:PD-L1 mRNA在不伴异常增生组、伴轻度异常增生组的表达水平分别较正常对照组上调1.3倍、1.7倍,但3组之间均无显著性差异(p>0.05)。PD-L2 mRNA在不伴异常增生组的表达水平较正常对照组上调3.0倍,两者之间有显著性差异(p<0.01);OLP伴轻度异常增生组的表达水平较正常对照组上调3.2倍,两者之间有显著性差异(p<0.01);OLP不伴异常增生组与伴轻度异常增生组之间无显著性差异(p>0.05)。结论:PD-L2可能在OLP的发病机制中起重要作用,而PD-L1可能并未参与OLP的发病。

Objective：To study the expression of PD-L1 and PD-L2 mRNA in lesions of oral lichen planus. Method：The expression of PD-L1 and PD-L2 mRNA in lesions of 30 OLP patients, which included 10 lesions with mild epithelial dysplasia and 20 lesions without epithelial dysplasia compared with normal mucosa of 10 healthy volunteers as controls,was examined by real-time PCR. Result：The levels of PD-L1 mRNA in lesions without epithelial dysplasia and with mild epithelial dysplasia was 1.3 times. 1.7 times more than that of normal mucosa respectively, but there was no significant difference between them （P 〉 0.05）. The levels of PD-L2 mRNA in lesions without epithelial dysplasia was 3.0 times more than that of normal mucosa, and there was significant difference between them （P 〈 0.01）;The levels of PD-L2 mRNA in lesions with mild epithelial dysplasia was 3.2 times more than that of normal mucosa, and there was significant difference between them （P 〈 0.01） ; and there was no significant difference between mild epithelial dysplasia group and without epithelial dysplasia group （P 〉 0.05）. Conclusion： PD-L2 may play an important role in pathogenesis of OLP, and PD-L1 may not be involved in onset of OLP.