加味三棱丸含药血清对子宫内膜异位症内膜细胞芳香化酶及环氧合酶-2的影响

Effects of medicated serum of SLW on aromatase and cyclooxygenase-2 in human endometrial cells of endometriosis in vitro

目的:观察子宫肌瘤与子宫内膜异位症(内异症)患者在位内膜芳香化酶(aromatase)和环氧合酶-2(cyclooxygenase-2,COX-2)表达上的差异,探讨加味三棱丸(SLW)含药血清对内异症在位内膜芳香化酶、COX-2表达的影响。方法:Western blot和RT-PCR方法分别检测子宫肌瘤组、给予SLW含药血清前后内异症组芳香化酶、COX-2蛋白和mRNA的表达,发光免疫分析法及放射免疫分析法分别检测上述各组细胞上清中雌二醇及前列腺素E2的含量。结果:以未给药的内异症内膜组为对照,子宫肌瘤组芳香化酶、COX-2 mRNA蛋白的表达,分泌雌二醇及前列腺素E2水平均明显低于对照组,差异有显著性(P<0.05)。SLW5.0,2.5g·kg-1·d-1组芳香化酶、COX-2蛋白和mRNA的表达低于对照组,差异有显著性或极显著性(P<0.05或P<0.01)。SLW1.25g·kg-1·d-1组芳香化酶mRNA和蛋白、COX-2蛋白的表达也明显低于对照组(P<0.01),但COX-2 mRNA的表达与对照组比较差异无显著性(P>0.05)。SLW5.0,2.5,1.25g·kg-1·d-1组分泌的雌二醇和前列腺素E2水平明显低于对照组(P<0.05)。结论:SLW可抑制子宫内膜异位症在位内膜细胞芳香化酶和COX-2的表达,从而降低局部雌激素及前列腺素E2的水平。

OBJECTIVE To investigate the difference in expression of aromatase and cyclooxygenase-2 in human eutopic en- dometrial cells derived from endometriosis and hysteromyoma and to explore the effect of medicated serum of SLW on aromatase and cyclooxygenase-2. METHODS After the model of eutopic primary cultured endometrial cells of endometiosis and hysteromyoma in vitro was successfully established, the changes of aromatase and cyclooxygenase-2 mRNA and protein were measured by RT-PCR and western blot respectively before and after treatment of medicated serum of SLW,and the level of secretion of E2 and prostaglandin E2 was also measured by ECLIA and RIA. RESULTS The expression of aromatase and cyclooxygenase mRNA, protein, secretion of estradiol and prostaglandin E2 in Hysteromyoma endometrium were weaker, as compared with that of eutopic primary cultured endometrial cells of endometiosis,which were taken as control group （P〈0.05）. After the treatment of medicated serum of 5.0,2.5 g.kg 1.d 1 of SLW, the expression of aromatase and cyclooxygenase-2 mRNA and protein in eutopic endometrial cells of endometriosis were found significantly lower than of the control group（P〈0.01 ）. Meanwhile, the expression of aromatase and cyclooxygenase-2 protein and aromatase mRNA in 1.25 g·kg^-1·d^-1 medicated serum group decreased,as compared with that of the control group（P〈0. 01）. However,The low dose group had no inhibitory effect on the expression of Cyclooxygenase-2 mRNA （P〉0. 05）. Compared with that of the control group, the level of the secretion of estradiol and prostaglandin E2 in cultural supernanant derived from endometrial cells of endometriosis in all three different dose of SLW groups was obviously lower（P〈0. 05）. CONCLUSION The medicated serum of SLW could inhibit the expression of aromatase and cyclooxygenase-2 in human eutopic endometrial cells derived from endometriosis and decrease the concentration of estradiol and prostaglandin E2 in local endometrium.