摘要
应用基因芯片技术考察了经频率为41.32 GHz的毫米波辐射的HL60白血病细胞和未经毫米波辐射的HL60白血病细胞组的基因表达差异,并用逆转录-聚合酶链式反应(RT-PCR)方法验证了IL-7、EGF和LGALS3基因变化.结果表明,毫米波辐射60min后,HL60细胞增殖,基因芯片检出基因表达上调18个和下调306个,在下调的基因中,RT-PCR检出IL-7、EGF和LGALS3基因下调的情况与基因芯片结果一致.这说明低功率毫米波可导致HL60细胞的基因表达谱发生变化,这些变化的基因与HL60细胞的增殖功能相关.
In this paper, the gene chip technology was adopted to reveal the difference in gene expression profile between the HL60 leukemia cells exposed to millimeter wave （MMW） at 41.32GHz and the sham-exposed group. The gene expressions of IL-7, EGF and LGALS3 were then confirmed by means of RTR-PCR method. The results show that, after the MMW radiation for 60 min, HL60 cells proliferate and 18 up-regulated genes and 306 downregulated genes are found, and that the down-regulated genes of IL-7, EGF and LGALS3 obtained by RT-PCR method are consistent with those obtained by gene chip technology. It is thus concluded that the radiation of MMW promotes the proliferation of HL60 cells by altering the gene expression profile.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2008年第4期133-137,共5页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省科技发展基金资助项目(2002C31101)
广东省自然科学基金资助项目(990587)
关键词
细胞
毫米波
辐射
基因表达
寡核苷酸
基因芯片
cell
millimeter wave
radiation
gene expression
oligonucleotide
gene chip
