新型碱性果胶酶用于棉针织物精练的工艺优化

Optimization of scouring of knitted cotton fabrics with a new-type alkaline pectinase

针对芽孢杆菌Bacillussp.WSHB04-02所产碱性果胶酶在棉织物精练中存在的问题,研究该碱性果胶酶应用于棉针织物精练的工艺条件,并进行优化。利用单因素法考察pH值、温度、酶用量和处理时间4个因素对酶精练效果的影响,得出该碱性果胶酶用于棉针织物精练的最优工艺条件为:酶活浓度960 U/L(1.2 mL/L),pH值7.0,温度60℃,处理时间40 min。对利用优化工艺处理的棉针织物的织物失重率、毛效、果胶去除率进行测定,与国外公司的相关产品以及传统碱精练工艺的使用效果做了比较。结果表明,新型碱性果胶酶具有中性、耐热性较好、成本较低和环境污染少的优点。

In order to solve the application problems of alkaline pectinase fromBacillussp.WSHB04-02 for scouring knitted cotton fabric,the conditions such as pH,temperature,enzyme concentrations and treatment time in bioscouring of knitted cotton fabrics with this pectinase were investigated and optimized in this work by use of″change-one-factor-at-a-time″method.The results were obtained as follows： pH=7.0;temperature,60℃;pectinase activity concentration 960 U/L;treatment time,40 min.Furthermore,comparisons were made with same kind of products from overseas as well as with the traditional alkaline scouring process in terms of the pectin removal rate,capillary effect and fabric weight loss.It was found that the new type enzyme has such advantages as neutrality,thermally stable,low cost and environmentally benign.