能量可控陡脉冲对兔VX2乳腺癌增殖和凋亡的影响

The Effect of Energy Controllable Steep Pulse on Proliferation and Apoptosis of Rabbit VX_2 Breast Tumor

目的:观察能量可控陡脉冲对荷瘤兔乳腺癌细胞增殖能力和凋亡的影响,分析陡脉冲对处理旁中心区域肿瘤组织潜在的影响。方法:实验兔24只制成VX2乳腺癌模型,随机分为处理组(n=21)和对照组(n=3),处理后0、12、24、48、72、120、168小时取肿瘤组织,通过HE染色、脱氧核糖核酸末端转移酶介导的dutp-生物素标记法(TUNEL法)、免疫组化观察处理中心组织及处理旁中心区域细胞形态、增殖指数(PI)及凋亡指数(AI)的改变。结果:陡脉冲处理后肿瘤中心凝固性坏死,旁中心区域见大量凋亡细胞,中心部分凋亡细胞较少。处理组细胞凋亡高峰在术后24小时左右,后逐渐下降,与对照组相比差异有显著性(P<0·05);PI随时间延长逐渐下降,与对照组相比差异有显著性(P<0·05)。结论:陡脉冲通过诱导处理旁中心区域肿瘤细胞凋亡,扩大陡脉冲处理的有效范围,促进肿瘤细胞的原位灭活。

Objective：To observe the change in the proliferating ability and apoptosis of VX2 breast cells induced by energy controllable steep pulse in rabbit tumor models, and to analyze the possible mechanism of the treatment of energy controllable steep pulse and the potential effect on the area around the center of treatment zone.Methods： The VX2 tumors were implanted subcutaneously in 24 rabbits. All animals were divided into two groups randomly： the ECSP group （ n=21 ） and the control group （ n=3）. The apoptosis index（AI） and the expression of PCNA index （PI） in the central tissue and the marginal tissue of the treatment zone in the implanted tumors were detected by HE staining, TUNEL, and immunohistochemistry staining at 0, 12, 24, 48, 72, 120, and 168 hours after treatment. Results：There were coagulative necrosis and apoptosis in the ECSP group. Many apoptotic cells could be seen in the area around the center of treatment zone, but few apoptotic cells in the center of treatment zone. In the control group, there were little TUNEL positive cells, the difference of AI between the ECSP group and the control group was significant （ P〈0.05）. An apoptotic peak could be seen at 24 hours in ECSP group, then decreasing gradually. In the ECSP group, the expression of PCNA decreased markedly at all time point after treatment. There were little PCNA positive cells at 168 hours in the tumors, the difference of PI between the ECSP group and the control group was significant（ P〈 0.05）.Conclusions： ECSP can extend the therapeutic area through inducing apoptosis and inhibiting proliferative ability in the tissue around the center of therapeutic zone and promote the destruction of the tumor in situ.