胎鼠心肌细胞凋亡调控基因bcl-2和bax的表达及意义

Expression of Bcl-2 and Bax in Myocardial Cells of Fetal Rat with Intrahepatic Cholestasis of Pregnancy

目的:探讨孕鼠肝内胆汁淤积症时胎鼠心肌细胞凋亡及调控基因bcl-2和bax的表达及意义。方法:应用雌孕激素建立妊娠期肝内胆汁淤积症大鼠模型,光镜观察胎鼠心脏的病理改变。采用原位末端标记法(TUNEL)和免疫组织化学方法检测妊娠第21天时胎鼠心肌细胞凋亡及调控基因bcl-2和bax的表达。结果:①肝内胆汁淤积症组(胆淤组)胎鼠心脏光镜下见心肌组织中心肌细胞空泡变性、水肿。②胆淤组胎鼠心肌细胞凋亡指数为39·79%±2·3421%,对照组为19·29%±2·4624%,两组比较,差异有非常显著性,P<0·01。③胆淤组胎鼠心肌细胞bcl-2基因的表达明显低于对照组,差异有非常显著性,P<0·01;胆淤组和对照组胎鼠心肌细胞bax基因的表达比较,差异无显著性,P>0·05。结论:孕鼠肝内胆汁淤积症时出现胎鼠心肌细胞凋亡,bcl-2基因表达下调可能参与调控胎鼠心肌细胞凋亡。

Objective：To investigate bcl-2 and bax expression and significance in fetal rat myocardial cells （FRM- Cs） with intrahepatic cholestesis of pregnancy （ICP）.Methods： The rat model of ICP was induced by 17-a- Ethinylestradiol and Progesterone. Pathohistological examinations of FRMCs were done under light microscope. TdT - mediated dUTP nick end labeling （TUNEL） and immunohistochemistry methods were used to detect apoptosis and bcl-2/bax expression in FRMCs of gestational 21st day. Results.①Under light microscope, there was vacuolar degeneration and edema in FRMCs in group ICP. ②The apoptosis index39.79% -2.3421% in FRMCs of group ICP was significantly higher than that 19.29% -2.4624% in control group, P〈0.01. ③The expression of bcl-2 protein in FRMCs of group ICP was significantly lower than that in control group, P〈0.01 ; There was no significant difference in the bax protein expression in FRMCs of two groups, P〉 0.05. Conclusions-It demonstrated that apoptosis appear in FRMCs of ICP. Low expression of bcl-2 protein in FRMCs may contribute to apoptosis in FRMCs of ICP.