12种翻白草种质资源遗传多样性的ISSR分析

Genetic diversity of 12 species of germplasm resources for Potentilla discolor based on ISSR analysis

目的 进行翻白Potentilla discolor种质资源的遗传多样性研究。方法 对12份翻白草种质进行ISSR分析。利用POPGENE 1.32软件分析遗传相似系数，UPGMA方法聚类，构建亲缘关系系统图。结果 12条引物共得到128条扩增条带，其中有101条呈现多态性，占78．91％。遗传相似系数变化范围0．1792～0．6325。聚类结果显示翻白草种质亲缘关系与地理分布有一定相关性，可以看出来源于同一地区的部分翻白草种质聚在一起，呈现出一定的地域性分布规律。结论翻白草的遗传多样性水平较高，种质间的亲缘关系和地理位置有一定关系。

Objective To study the genetic diversity of germplasm resources for Potentilla discolor.Methods Twelve species of germplasm resources for P. discolor were analyzed by ISSR molecular markers. To make up the systematic diagram of genetic relationship by POPGENE 1.32 software, cluster by UPGMA method, and establish the dendrogram. Results A total of 128 ISSR bands were scored for 12 primers, among which 101 were polymorphic bands. The average percentage of polymorphic bands was 78.91%. Genetic similarity coefficient was changed from 0. 179 2 to 0. 632 5. By cluster analysis, thegeographical distribution is mutually related to the relationship of germplasm resources for P. discolor and it was also showed some of P. discolor from the same region were in the same group which presented therule of geographical distribution in the tested materials. Conclusion The diversity level of the different germplasm resources for P. discolor higher and the relationship of P. discolor correlates with the geographical location in some way.