广西莪术试管内诱导根状茎形成的研究

Rhizome plantlet formation of Curcama kwangsiensis

目的提纯复壮广西莪术种质资源,提高良种组培苗移栽成活率,方便组培苗生产的贮藏和管理。方法调节MS培养基中大量元素和蔗糖的量,使组培苗在试管内直接诱导广西莪术小根状茎的形成。结果培养基中大量元素和蔗糖的量是试管内直接诱导广西莪术小根状茎形成的重要影响因素。以1/2 MS和6%蔗糖结根状茎率最高,根状茎最粗,组培苗壮且根系发育良好,直接移栽的成活率最高。结论能够在试管内诱导广西莪术形成根状茎。

Objective To purify and rejuvenate the plant of Curcama kwangsiensis, improve thetransplantation survival rate of cultivars, and make the convenience of storagement and management for the cultivars production. Methods The rhizome plantlet formation of C. kwangsiensis was induced by regulating both of macro-element and sucrose concentration in the MS culture medium. Results Both ofthem were very important influence factors on the rhizome plantlet formation of C. kwangsiensis. Media 1/2 MS and 6% sucrose were adaptable to the rhizome plantlet formation of C. kwangsiensis, had the besteffects on the formation rate of the rhizome plantlet, diameter and direct transplanting rate of the rhizome plantlet. Both plantlet and root system development were stronger. Conclusion In the present research, it is successful in inducing the rhizome plantlet formation of C. kwangsiensis.