水杨苷异羟肟酸和茉莉酸甲酯对唐菖蒲试管结球的影响

Effects of Salicylhydroxamic Acid (SHAM) and Methyl Jasmonate (MJ) on in Vitro Corm Formation of Gladiolus hybridus

为探讨水杨苷异羟肟酸(SHAM)和茉莉酸甲酯(MJ)对唐菖蒲试管结球的影响,以‘RoseSupreme’的籽球为外植体,研究了不同处理球茎内可溶性蛋白、内源MJ、碳水化合物(蔗糖、淀粉和纤维素)含量、脂氧合酶(LOX-1)、抗氧化酶(SOD、CAT、POD和APX)活性和LOX同工酶变化与试管结球之间的关系。结果表明:与对照相比,SHAM处理使结球时间明显推迟,结球率、球茎鲜样质量和直径降低;可溶性蛋白含量、LOX-1活性和MJ含量显著降低,LOX同工酶谱带变弱,并有2条同工酶带逐渐消失;抗氧化酶活性和碳水化合物含量降低。而MJ处理后上述指标均不同程度上调,0.5mmol.L-1为测试浓度范围内促进唐菖蒲试管结球的最佳浓度。以上试验结果说明SHAM对唐菖蒲试管结球起抑制作用,而MJ有明显的促进作用,可能通过外施MJ提高了LOX活性及内源MJ含量,调动碳水化合物的积累,增加抗氧化酶的活性,从而对球茎的发生和生长进行调控。

To investigate the effects of SHAM and MJ in vitro on corm formation in Gladiolus hybridus ＇Rose Supreme＇ , the contents of total soluble protein, endogenous methyl jasmonate （M J） and carbohydrates as well as the activities of LOX-1 and antioxidant enzymes were examined under different treatments. LOX isozymes were also analysized. The results showed that, under the SHAM treatment, corm formation was obviously delayed and reduced in terms of frequency and corm fresh mass and diameter and that the concentrations of total protein, MJ and carbohydrates, and the activities of antioxidant enzymes and LOX-1 were all reduced compared to the control. The activities of LOX isozymes became weaker with two isozyme bands disappeared. In contrast, these physiological and biochemical indexes were increased under MJ treatments, in which, 0. 5 mmol ·L^-1 was the optimal concentration for promoting corm formation in Gladiolus. These results suggested that corm formation could be improved by MJ but suppressed by SHAM through regulating the LOX activity, resulting in carbohydrate accumulation and increase of antioxidant enzymes activities in Gladiolus hybridus.