FT-IR、UV/VIS、Raman光谱法研究血红蛋白和银、铜离子的作用

The Investigation for Interaction of Hemoglobin with Silver (Ⅰ) and Copper (Ⅱ) Ions by FT-IR, UV/VIS and Raman Spectroscopies

本文报导血红蛋白（Hb）同银（Ⅰ）-铜（Ⅱ）离子的作用．研究表明，在pH10．5的B5O72--OH-缓冲介质中，当有十二烷基硫酸钠（SDS）和甲醛存在时，Hb－Ag-Cu物种表现出强的橙黄色，用FT-IR、UV／VIS和振动Raman光谱研究了Hb-Ag-Cu物种的形成机理．表明血红蛋白中氨基酸残基上的氮和氧与银-铜离子有较强的作用，对Hb中氨基的修饰证明了这一机理。

The interaction on hemoglobin (Hb) with silver(I)-copper (II) ions have been developmented byFT-IR,UV /VIS,Raman spectroscopies. It's shown that the Hb-Ag-Cu species are appear to strongerorange-yellow colour when sodium dodecyl sulphate (SDS) and formaldehyde have presence and thepH 10. 2 range of B4O72--OH- buffer. It is forming a sensitive species that the silver and copper ionswith nitrogen and oxygen atums of residues of amino acid in Hb. The mechanism was proved that bymodification of amino groups.