氟喹诺酮类药物与牛血清白蛋白作用的研究

Studies on the Interaction between BSA and Fluoroquinolones

用荧光猝灭法研究了ＢＳＡ与ＮＦＬＸ及ＯＦＬＸ之间的相互作用．根据Ｆｒｓｔｅｒ的能量转移理论，确定了能量给体－受体间的距离．根据荧光猝灭光谱绘制了猝灭曲线，确定其猝灭过程是静态猝灭．用Ｌｉｎｅｗｅａｖｅｒ－Ｂｕｒｋ双倒数曲线测定了ＮＦＬＸ－ＢＳＡ和ＯＦＬＸ－ＢＳＡ络合物的离解常数，并详细研究了１０种阳离子、４种阴离子与药物对ＢＳＡ竞争结合的影响，推测了药物与蛋白的主要作用力．

The interaction between BSA and NFLX or OFLX were studied by means of fluorescence quenching. According to Forsters theory, the distances between the energy donor and acceptor were determined. The quenching curves were obtained by using the fluorescence quenching spectra, which showed the quenching were static quenching. The dissociation constants of NFLX BSA and OFLX BSA were determined by using the Lineweaver Burk curves. The effect of competition between the drugs and 10 cations or 4 anions for BSA were investigated in detial. The chemical attraction of NFLX BSA and that of OFLX BSA were inferred.