孕血和药物中孕酮的单扫极谱法测定

Single sweep Oscillopolarography Determination of Progesterone in Pregnancy Plasma and Pharmaceutical Preparations

孕酮２，４－二硝基苯腙在单扫描示波极谱仪上，以吡啶－氢氧化钠为底液（ｐＨ１２．０），于－０．７４Ｖ（ｖｓ．ＳＣＥ）处可出现一灵敏的吸附催化波．孕酮浓度在１．０×１０－８～２．０×１０－６ｍｏｌ／Ｌ范围与该波呈线性关系，检出限为５．０×１０－９ｍｏｌ／Ｌ．方法用于测定孕血和药物中孕酮含量，获得满意结果．

By using single sweep oscillopolarography, the progesterone 2,4 dinitrophenylhydrazone can produce a sensitively polarographic catalysis wave in pyridine sodium hydrate media at pH12.0 at -0.74V(vs.SCE). The peak height is linear with progesterone concentration from 1.0×10 -8 mol/L to 2.0×10 -6 mol/L and the detection limit can reach 5.0×10 -9 mol/L. The method has been satisfacltorily applied to determine trace amounts of progesterone in pregnancy plasma and pharmaceutical preparations.