荧光胺与芳伯胺衍生反应的介质效应

MEDIUM EFFECTS ON DERIVATIVE REACTION BETWEEN FLUORESCAMINE AND PRIMARY ARLYAMINE

研究了ｐＨ、多种有机溶剂及其用量、表面活性剂、环糊精等介质环境对荧光胺与对氨基苯磺酸衍生反应及其产物荧光特性的影响。该衍生反应需要有机溶剂，多种有机溶剂均可使用，但用量宜少。所试验的各类表面活性剂中，仅ＴｒｉｔｏｎＸ－１００有显著的增敏作用，且其增敏效应受有机溶剂用量的影响，显示出明显的竞争效应。

The effects of pH,various organic solvents,surfactants,β CD and PVA on derivative reaction and fluorescence properties of its product between fluorescamine and p amino phenyl sulfonic acid have been studied The results show that the derivative reaction needs the organic solvent,many kind of organic solvents are available,but its amount should not be too large The rate of derivative reaction was slowed and fluorescence intensity of product was decresed with increase in amount of organic solvent and precision is not good because of the fluorescence intensity is changing with delay time Only tritonX 100 enhanced the fluorescence of product in all of the surfactants tested Span20,Tween80 and Brij35which same as tritonX 100 are non ionic surfactant,and PVA,β CD do not exhibit sensitization Ionic Surfactant CTAB,SDS quench the fluorescence The sensitization of tritonX 100 is dependent on the amount of organic solvent A competitive effect between tritonX 100 micellae and organic solvent is exhibition In this paper,the derivative reaction between fluorescamine and p chloroaniline is compared and studied too