时间分辨光谱法用于离体人肺鳞癌、腺癌及正常组织的荧光寿命测量

FLUORESCENCE LIFETIMES OF SQUAMOUS CARCINOMA,ADENOCARCINOMA AND NORMAL HUMAN LUNG TISSUES IN VITRO BY TIME RESOLVED FLUORESCENCE

用Ｎｄ∶ＹＡＧ倍频光５３２．０ｎｍ泵浦ＤＣＭ染料输出的６３２．８ｎｍ激光作激发源，通过时间分辨法测得离体人肺腺癌、鳞癌及正常组织的特征峰荧光寿命分别为５．１，５．６和６．３ｎｓ。实验结果表明：肿瘤组织的荧光寿命总小于正常组织的荧光寿命。

Using pulsed dye laser 632 8nm,lifetimes of adenocarcinoma,squamous carcinoma and normal human lung tissues in vitro have been measured by time resolved fluorescence as 5 1,5 6 and 6 3 ns respectively.Time resolved fluorescence shows that the decay time of tumor tissues is faster than that of normal tissues.The results may offer new diagnostic method to determine cancer.