利用NaOH碱式消化快脉冲雾化原子吸收法测定培养细胞内的K、Ca和Mg含量

DETERMINATION OF CALCIUM MAGNESIUM AND POTASSIUM IN NURTURED CELL BY AAS WITH QUICK PULSED NEBULIZATION TECHNIQUE AND NaOH BASE DIGESTION

用６％的ＮａＯＨ碱式消化培养细胞，取５０μｌ消化液，用考马斯亮蓝法［１］测定蛋白含量，其余用Ｌａ－ＥＤＴＡ等量稀释后，采用自制微量进样装置，运用快脉冲雾化技术［２］，使进样体积最低降至２０μｌ，在日立１８０－８０型原子吸收分光光度计上测定Ｋ、Ｃａ和Ｍｇ含量。

The sample is digested with 6% NaOH solution and an amount of 50μl is used for protein content analysis by the method of Comassie Brilliant Blue G250,the residual is diluted with equal 0 4% Lathanurm EDTA solution.Its Calcium magensium and potassium content are determined by AAS.With quick pulsed nebulization technique.When a self made micro sampling device is used,20μl of sample volume is needed and it is only the 1/10～1/20 of the sample volume required for conventional determination.Sensitivity,precision and rate of recovery agree well with those using regular wet ashing method.