萃取-火焰原子吸收法测定骨骼中的微量元素Cu、Zn、Cd和Fe

EXTRACTION FLAME ATOM ABSORPTION DETERMINATION OF TRACE COPPER,ZINC,CADMIUM AND IRON IN BONES

本文研究了以邻菲罗啉为金属螯合剂，高氯酸钠为配体，在一定酸度下用１，２－二氯乙烷萃取－火焰原子吸收法测定动物骨骼中微量Ｃｕ、Ｚｎ、Ｃｄ和Ｆｅ的条件，并确定了测定方法。方法简单、准确、可靠。元素的检测限分别为：Ｃｕ：０．０３８μｇ／ｍｌ，Ｚｎ：０．００４２μｇ／ｍｌ，Ｃｄ：０．００１９μｇ／ｍｌ，Ｆｅ：０．０２μｇ／ｍｌ，回收率在９２．

Trace content of Cu,Zn,Cd and Fe in bones is determined by flame atomic absorption spectrometry after preceding preconcentration using the extraction system O phenanthroline sodium perchlorate/1,2 dichloroethane.Also,the factors of influence were studied.The method was simple,accurate and reliable,The detection limits were Cu:0 038μg/ml,Zn:0 0042μg/ml,Cd:0 0019μg/ml,Fe:0 02μg/ml.The recovery rate was between 92 8 and 105%.