泥炭、软褐煤中的腐殖质的荧光光谱研究

CATALYTIC FLUORIMETRIC DETERMINATION OF TRACE AMOUNT OF BROMINE ION

对滇西镇安盆地泥炭、软褐煤中的腐殖质进行了荧光光谱研究，发现它们的荧光性质很复杂，有些腐殖质可以象稳定组分一样发出橙黄至黄色荧光，Ｉ５４６＝８．１３～１３．１７，单峰或不完全双峰，λｍａｘ＝５６０～６０５ｎｍ，Ｑ６５０５００＝０．６０～２．９３；大部分腐殖质不发荧光或发暗棕褐色荧光，Ｉ５４６＝０～５．３８，因荧光太弱而难以获得荧光光谱。但被激发数分钟后，有些不发荧光或发暗棕褐色荧光的腐殖质其荧光会渐渐强烈起来，发黄褐至暗橙黄色荧光，Ｉ５４６＝６．１２～１２．１７，λｍａｘ＝５９０～６２５ｎｍ，Ｑ６５０５００＝３．４９～４．３１；随激发时间进一步延长（１０～２０分钟），有些腐殖质甚至会象稳定组分一样发出比较强烈的黄色荧色，Ｉ５４６＝１２．１８～２０．０１，单峰，λｍａｘ＝５５５～５９５ｎｍ，Ｑ６５０５００＝０．８７～１．６０。

A new catalytic fluorimetric method for the determination of bromine ion based on its catalytic effect on the oxidation of fluorescein by potassium bromate in the solution of dilute sulfuric acid has been established.The detection limit for Br - is 1 94×10 -2 μg/25mL.The linear range of the determination is 6 0～12 0μg/25mL.The method has been applied to the determination of Br - in tablet pyridostigmini bromidum sample with satisfactory results.