摘要
The objective of the present study was to prepare a novel nanostructured surface of Ti6Al4V alloy by the severe plastic deformation (SPD) and the chemical treatment process and to evaluate the adhesion of osteoblast on the nanophase titanium alloy. In the in vitro study, the primary cultured osteoblasts of neonatal rat calvaria were cultured on the nanophase and the as-received smooth Ti6Al4V substrates. Then osteoblasts adhesion behaviors on different substrates were observed by the fluorescence mi-croscopy, scanning electron microscopy (SEM) and RT-PCR analysis. The results of our research showed increased osteoblast adhesion on the nanophase titanium alloy compared with the as-received case. On the nanophase substrate, the presence of extensive filopodia, strong cellular adhesion and early cellular confluency could be observed. In addition, the expression of the adhesion-related integrin β1 mRNA was also higher on the nanophase substrate. It suggested that the nano technology could be further considered for orthopedic implant applications.
The objective of the present study was to prepare a novel nanostructured surface of Ti6Al4V alloy by the severe plastic deformation （SPD） and the chemical treatment process and to evaluate the adhesion of osteoblast on the nanophase titanium alloy. In the in vitro study, the primary cultured osteoblasts of neonatal rat calvaria were cultured on the nanophase and the as-received smooth Ti6AI4V substrates. Then osteoblasts adhesion behaviors on different substrates were observed by the fluorescence microscopy, scanning electron microscopy （SEM） and RT-PCR analysis. The results of our research showed increased osteoblast adhesion on the nanophase titanium alloy compared with the as-received case. On the nanophase substrate, the presence of extensive filopodia, strong cellular adhesion and early cellular confluency could be observed. In addition, the expression of the adhesion-related integrin β1 mRNA was also higher on the nanophase substrate. It suggested that the nano technology could be further considered for orthopedic implant applications.
基金
the Shanghai Nano Funding (Grant No. 0452nm061)
