高效液相色谱法和因子分析-紫外光度法测定荧光增白剂中两种成分

Determination of the Two Components in Fluorescent Whitening Agent by HPLC and Factor Analysis UV Spectrophotometry

本文采用两种同时测定荧光增白剂工业产品中１，４－（２，４′－氰基苯乙烯基）苯（记为ＥＢ）和异构体１，４－双（２－氰基苯乙烯基）苯（记为ＥＲ）两组分的方法。一种是ＨＰＬＣ法，采用Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＳＩＬ柱（５μｍ，６．０ｍｍｉ．ｄ．×１５０ｍｍ），正己烷－乙酸乙酯（９０∶１０Ｖ／Ｖ）为流动相，流速２ｍＬ／ｍｉｎ，柱温３０℃，检测波长ＵＶ３６０ｎｍ，方法简单、准确、快速。另一种是因子分析－紫外光度法，它对吸收光谱严重重叠的该增白剂产品中两组分的例行分析非常适用，效果较好，测其所含的ＥＢ、ＥＲ两组分的回收率均在１００±８％之内。

An HPLC method and a factor analysis UV spectrophotometric method were applied to simultaneous determination of the two components in fluorescent whitening agent,i.e.,1,4 (2,4′ dicyano bis styryl) benzene (EB) and its isomer 1,4 bis(2 cyanostyry) benzene (ER).The HPLC stationary phase was Shim pack CLC SIL (5μm,6.0mm i.d.×150mm) and the mobile phase consisted of 90∶10( V/V ) n hexane ethyl acetate at a flow rate of 2mL/min.The column temperature was kept at 30℃.The wavelength of UV detector was set at 360nm.The method is simple,accurate and rapid.The factor analysis method is especially suitable for routine analysis of samples of which the absorption spectra of the components are severely overlapped .The recoveries of the two components were within 100±8%