高效液相色谱法测定小麦幼苗中植物生长调节剂

Determination of Plant Growth Regulater in wheat Seedlings by High Performance Liquid Chromatography

介绍了高效液相色谱测定麦苗中植物生长调节剂（赤霉素（ＧＡ３），３－吲哚乙酸（ＩＡＡ））的方法，用国产预处理小柱处理样品。并以甲醇∶１％冰乙酸＝１２∶８８（Ｖ／Ｖ）为流动相，色谱柱为ＺｏｒｂａｘＣ１８，在２１０ｎｍ紫外检测。

A high performance liqid chromatographic method was developed for determining the quantity of gibberellin(GA 3) and 3 indole acetic acid(IAA) in wheat seedlings .The wheat seedlings were homogenized in 80% methanol in a mixing vessel for 24hr at 4℃,filtered and the residue reextracted with 80% methanol for three times.The combined filtrate was evaporated to a small volume (20mL) under vacuum at 40℃(Water bath) and subsequently purified by forcing the solution through a PT C 18 cartridge with the aid of a 10mL syringe to remove the non polar compounds from the solution. The filtrate was then furtherreduced in volume (5mL) under vacuum at 40℃ and again forced through a PT C 18 cartridge .The filtrate was subsequently evaporated to dryness and taken up in 2mL of the mobile phase for reversed phase high performance liquid chromatography. It is essential that the H of the sample be adjusted to acidic.