非小细胞肺癌中VEGF-C、PDGF-BB与淋巴管生成和淋巴结转移的关系

Relationship among VEGF-C,PDGF-BB,lymphangiogenesis and lymph node metastasis in human non-small cell lung cancer

目的:探讨血管内皮生长因子C(VEGF-C)和血小板衍生生长因子BB(PDGF-BB)在非小细胞肺癌(NSCLC)中的表达与淋巴管生成和淋巴结转移的关系。方法:分别应用免疫组化SP法和EnvisionSystem法检测40例NSCLC和10例肺良性病变组织中VEGF-C和PDGF-BB的表达;应用淋巴管特异标志Podoplanin检测NSCLC中淋巴管密度(LVD),并做相关统计分析。结果:VEGF-C和PDGF-BB在NSCLC中阳性表达率均显著高于肺良性病变(VEGF-C:62.5%vs10%,P<0.05;PDGF-BB:60%vs10%,P<0.05)。淋巴结阳性组的VEGF-C阳性表达率显著高于淋巴结阴性组(94.1%vs39.1%,P<0.05),但未发现PDGF-BB表达与淋巴结有关(76.5%vs47.8%,P>0.05)。NSCLC中淋巴结阳性组的LVD显著高于淋巴结阴性组(16.58±2.38vs9.88±1.93,P<0.05),VEGF-C和PDGF-BB阳性表达组的LVD均显著高于阴性表达组(VEGF-C:14.74±3.62vs9.37±1.35,P<0.05;PDGF-BB:13.84±4.23vs11.06±2.90,P<0.05)。NSCLC中VEGF-C与PDGF-BB的表达具有显著相关性(rs=0.422,P<0.05)。结论:淋巴管生成是淋巴结转移的一个重要因素;PDGF-BB参与了淋巴管生成,但对于促进淋巴结转移可能不是必要的;VEGF-C通过参与淋巴管生成而促进淋巴结转移,其预测NSCLC发生淋巴结转移可能性的价值优于PDGF-BB;PDGF-BB和VEGF-C在促进淋巴管生成的作用上可能具有相关性。

Objective：To explore the relationship among vascular endothelial growth factor C（VEGF-C）,platelet-derived growth factor B B （PDGF-BB）,lymphangiogenesis and lymph node metastasis in human non-small cell lung cancer（NSCLC）. Methods：Forty cases of NSCLC with complete follow-up information and ten cases of lung benign diseases were included. Tumor samples were immunostained for vascular endothelial growth factor-C （VEGF-C）, platelet-derived growth factor BB （PDGF-BB） and the lymphatic endothelial markers Podoplanin. Lymphatic vessel density （LVD） was evaluated within NSCLC. Results：Both of the positive rate of VEGF-C and PDGF-BB in the human NSCLC group were significantly higher than that in the lung benign diseases group（VEGF-C：62.5% vs 10%, P 〈 0.05 ;PDGF-BB：60% vs 10% ,P 〈 0.05）. In NSCLC the positive rate of VEGF-C in the positive lymph node group was significandy higher than that in the negative lymph node group （94.1% vs 39.1%,P 〈 0.05）. However, no significant correlation was found between the expression of PDGF-BB and lymph node status （76.5% vs 47.8%,P 〉 0.05）. In NSCLC the LVD in the positive lymph node group was significantly higher than that in the negative lymph node group（16.58 ± 2.38 vs 9.88 ± 1.93,P 〈 0.05）. LVDs in the positive VEGF-C and PDGF-BB group were significantly higher as compared with the negative expression group（VEGF-C： 14.74 ± 3.62 vs 9.37 ± 1.35 ,P 〈 0.05 ;PDGF-BB： 13.84 ± 4.23 vs 11.06 ± 2.90,P 〈 0.05）. Furthermore,there was significant correlation between VEGF-C and PDGF-BB in human NSCLC （rs = 0.422,P 〈 0.05）. Conclusion：Lymphangiogenesis is a significant factor for tumor lymphatic metastasis. PDGF-BB may not be necessary for lymphatic metastasis in human NSCLC despite mediating lymphangiogenesis. VEGF-C may accelerate lymphatic metastasis in human NSCLC by inducing lymphangiogenesis,and it may be more valuable than PDGF-BB for forecasting the status of lymphatic metastasis in human NSCLC. There might be similar mechanism between VEGF-C and PDGF-BB on mediating lymphangiogenesis.