Fas(TNFRSF6)基因位点A670G多态性与子痫前期的相关性研究

A670G polymorphism at position-670 in the Fas gene in pregnant women with pre-eclampsia

目的:探讨Fas(TNFRSF6)基因位点A670G多态性与子痫前期(pre-eclampsia)的相关性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对140例子痫前期患者(轻度子痫前期75例,重度子痫前期65例)和170例同期入院产检并在南京医科大学第一附属医院分娩的正常孕妇,进行Fas(TNFRSF6)基因位点A670G多态性的分析,比较各基因型及等位基因的频率在病例组和正常组间、轻度子痫前期组和重度子痫前期组间的差别。结果:G等位基因型频率在子痫前期组(56.4%)显著高于对照组(36.2%)(P<0.001),G等位基因型频率在重度子痫前期组(64.6%)显著高于轻度子痫前期组(49.3%)(P=0.010)。结论:Fas(TNFRSF6)基因位点A670G多态性的G等位基因可能是子痫前期的重要遗传危险因素,并与病情的严重程度相关。

Objective：To estimate whether A670G polymorphism at position-670 in the gene coding for Fas（gene symbol TNFRSF6） is associated with pre-eclampsia. Methods：By means of polymerase Chain Reaction and Restriction Fragment Length Polymorphism, A670G polymorphism at position -670 in the Fas（TNFRSF6） gene was detected in 140 women with pre-eclampsia（75 with mild preeclampsia and 65 with severe pre-eclampsia） and 170 normal control women with uncomplicated pregnancy. Investigators were blinded to clinical outcomes. Results： G Allele frequency in pre-eclampsia group were significantly higher than those in control group （56.4% vs 36.2%）（P 〈 0.001）. G Allele frequency in severe pre-eclampsia group were significantly higher than those in mild preeclampsia group（64.6% vs 49.3%）（P = 0.010）. Condusion：The G Allele of Fas（TNFRSF6） gene might be a risk genetic marker of pre-eclampsia. In patients with pre-eclampsia, there is the association between G Allele of Fas （TNFRSF6） gene and severity of preeclampsia.