IL-1B及IL-1RN基因多态性与江苏地区宫颈癌遗传易感性相关

Associations between functional genetic polymorphisms of IL-1B and IL-1RN and risk of cervical cancer

目的:探讨炎症因子IL-1B(T-31C和C-511T)及其拮抗基因IL-1RN基因多态性与宫颈癌遗传易感性的关系。方法:采用病例对照研究,对经组织学确诊的宫颈癌新发病例273例按年龄和城乡频数匹配正常对照402例,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行多态性检测,比较不同基因型与宫颈癌发病风险的关系。结果:在调整年龄、城乡等因素后,携带-511CT基因型比携带CC基因型个体发生宫颈癌的危险性增加93%(调整OR=1.93,95%CI=1.00～3.72);携带-511TT基因型比携带-511CC基因型个体发生宫颈癌的危险性增加115%(调整OR=2.15,95%CI=1.02～4.53)。分层分析表明,携带IL-1BT-31C变异基因型的个体发生宫颈癌的风险仅在年龄≥50岁(调整OR=1.72,95%CI=1.01～2.93)、妊娠次数≥2次(调整OR=1.83,95%CI=1.07～3.11)和农村地区女性(调整OR=1.63,95%CI=1.04～2.54)中显著。IL-1RN各基因型在病例组和对照组中分布的差异均无统计学意义。结论:IL-1BC-511T多态性可能与江苏地区妇女的宫颈癌易感性相关。

Objective：To study the relationship between functional genetic variants of IL-1B （T-31C,C-511T） and IL-1RN genes and the susceptibility of cervical cancer. Methods：The case-control study was conducted in Jiangsu female population,including 273 cervical cancer cases and 402 cancer-free controls frequency-matched by age and residential areas. Genotypes were determined by PCR-the restriction length polymorphism（PCR-RFLP） assays. Results： The differences of IL-1B T-31C and C-511T genotype frequen- cies between cases and controls were not statistically significant. After adjusted age and residences, the Logistic regression analysis revealed that the increased risk of cervical cancer was associated with IL-1B -511TT and -511CT variant genotypes （OR=1.93,95% CI= 1.00-3.72 for CT genotypes ; OR=2.15,95% CI=1.02-4.53 for TT genotypes）, compared with the -511CC genotype. Stratified analysis indicated that IL-1B T-31C variant genotypes were statistically associated with cervical cancer risk in the subjects with more than 49 years old （OR=1.72,95%CI=1.01-2.93）,pregnancy frequency ≥2 （OR=1.83,95%CI=1.07-3.11） and living in countryside （OR= 1.63,95%CI=1.04-2.54）. No significant association was observed between polymorphisms of IL-1RN gene and cervical cancer risk. Conclusion：These findings supported the hypothesis that the functional genetic variants of IL-1B C-511T might contribute to the cervical carcinogenesis in Jiangsu population.