黄芩苷对STZ糖尿病大鼠肾脏的保护及其与VEGF的关系

The protective effect of baicalin on the kidney of STZ induced diabetic rats and its relation to VEGF

目的:探讨黄芩苷对糖尿病大鼠肾脏病变的影响。方法:将实验大鼠随机分为3组,正常对照组(A)、糖尿病对照组(B)和糖尿病黄芩苷灌胃组(C),所用黄芩苷剂量为200mg.kg-1。实验为期4周,观察血糖、糖化血红蛋白、体质量/肾质量比和尿白蛋白排泄率,用免疫组化法检测肾小球血管内皮生长因子(VEGF)的表达。结果:1.糖尿病大鼠血糖和糖化血红蛋白增高、肾质量/体质量比增加,肾小球节段性系膜细胞增生,肾小管空泡变性,少数肾小管上皮细胞坏死,肾小球VEGF表达增加;2.C组和B组相比血糖无明显变化,肾质量/体质量比无明显变化,但糖化血红蛋白明显降低,尿白蛋白排泄率降低,肾小球无明显异常,肾小管上皮轻度空泡变性,VEGF表达减少。结论:本实验初步研究表明,黄芩苷对STZ糖尿病大鼠肾脏病变具有一定的保护作用,其机理可能与抑制蛋白糖化及VEGF表达减少有关。

OBJECTIVE To investigate the influences of baicalin on the kidney of STZ induced diabetic rats and its relation to VEGF. METHODS The rats were randomly divided into three groups, （ 1 ） normal controls （A） ; （2） diabtetie controls （B） ; （3） diabetic treated group （C） by stomachtube, dosed by baicalin 200 mg·d ^-1. The duration of test was 4 weeks. The observationparamelers included blood glucose, HbA1 c, kidney weight/ body weight ratio, urine albumin exretion rate （UAER）, vascular endothelial growth factor （VEGF） expression in glomeruli by immunohistochemic method. RESULTS 1. In diabetic controls （B group ） blood glucose and HbA1c level was significantly elevated,and the ratio of kidney weight/body weight was increased, glomerular segmental mesangial cell hyperplasia, renal tubulus cell vacular degeneration and necrosis. VEGF expression increased in glomeruli. 2. In C group , the blood glucose and the ratio of kidney weight/body weight were not significandtly different from B group, but the HbA, c and UAER were significantly decreased, the kidney had no significant pathologic change, VEGF expression in glomeruli was diminished. CONCLUSION Baicalin has protective effect on the kidney of diabetic rats induced by STZ,baicaiin may inhibite protein glucosylation and inhibite glomerular VEGF expression.