气相-质谱联用法测定维生素E胶丸中维生素E的含量

Determination the content of vitamin E in vitamin E soft capsules by GC-MS

目的:建立了使用气相-质谱联用仪,采用选择监测离子法测定维生素E胶丸中维生素E的含量。方法:DB-5MS色谱柱(30m×0.32mm,0.25μm),氦气为载气,质谱为检测器,乙醇为溶剂,直接进样,选择检测离子m/z:430,外标法计算维生素E的含量。结果:维生素E在0.01～10mg.L-1范围内线性良好(r=0.9998,n=6);平均回收率为100.3%,RSD为1.06%(n=5),检测限在10pg。结论:本方法简便、快速、灵敏度高、结果准确可靠,可用于该制剂的质量控制。

OBJECTIVE To establish a method for determining the content of vitamin E in vitamin E soft capsule by GC/ MS. METHODS The GC/MS was equipped with a mass detector, A chromatographic column （30 m× 0. 32 mm,0. 25 μm） with DB-5MS was adopted. The carrier gas was helium. The m/z was 430. The assay of vitamin E was calculated according to the external standard method. RESULTS Vitamin E is detected in the sample of vitamin E soft capsule. The standard curves were linear within the ranges of 0. 01-10 mg· L ^-1 （r = 0. 9998,n = 6）. The average recoveries were 100. 3 % （RSD = 1. 06 %,n =5）. The limits of detection were 10 pg. CONCLUSION This method appears to be sensitive, accurate, repeatable,easy to operate and can be used for the quality control of vitamin E soft capsules.