鼻咽癌HSP70表达与血清EB病毒IgA/VCA的关系及其临床意义

Correlation of Heat Shock Protein 70 Expression in Nasopharyngeal Carcinoma to Immunoglobin A against Viral Capsid Antigen of Epstein-Barr Virus in Sera and Its Clinical Significance

背景与目的:热休克蛋白(HSP)70在许多恶性肿瘤中均有表达,但在鼻咽癌组织中的表达与血清IgA/VCA滴度及预后的关系尚不清楚。本研究旨在检测鼻咽癌组织中HSP70的表达和含量水平,探讨HSP70表达与鼻咽癌患者血清EB病毒IgA/VCA滴度及预后的关系。方法:采用SP免疫组化法检测38例鼻咽癌组织中HSP70表达,ELISA法检测38例鼻咽癌组织中HSP70含量,免疫酶标法检测38例鼻咽癌患者血清IgA/VCA滴度。结果:HSP70在38例鼻咽癌组织中表达率为60.5%。在不同性别、年龄、T分期、N分期和临床分期鼻咽癌组织中HSP70表达率均无显著性差异(P>0.05)。HSP70表达和含量与血清EB病毒抗体IgA/VCA滴度呈正相关(P=0.001)。HSP70阳性组和阴性组患者的5年生存率分别为65.2%和80.0%,5年无瘤生存率分别为40.0%和78.6%(P=0.04)。结论:HSP70在临床Ⅱ、Ⅲ期鼻咽癌患者癌组织中的表达与患者的IgA/VCA滴度正相关,HSP70阳性患者常规治疗后预后较差。

BACKGROUND ＆ OBJECTIVE： Overexpression of heat shock protein （HSP）70 is expressed in many tumors, but the correlation of its overexpression in nasopharyngeal carcinoma （NPC） to immunoglobin A against viral capsid antigen of Epstein-Barr virus （EBV） in sera and its clinical significance are still unclear. This study was to determine the expression of HSP70 in NPC, and to analyze its correlations to EBV IgA/VCA titer and prognosis. METHODS： The expression of HSP70 in 38 specimens of stage Ⅱ -Ⅲ NPC was determined by SP immunohistochemistry; the content of HSP70 in the 38 specimens was detected by ELISA; the EBV IgA/VCA titer in sera of the 38 patients was detected by immunoenzymatic （IE） method. RESULTS： The positive rate of HSP70 in the 38 NPC samples was 60.5%. HSP70 expression was positively correlated to EBV IgA/VCA titer （r=0.690, P=0.001）, but not to sex, age, and clinical stage （P〉0.05）. The 5-year survival rates of HSP70-positive group and HSP70-negative group were 65.2% and 80.0%. The 5-year tumor-free survival rates of HSP70-positive group and HSP70-negative group were 40.0% and 78.6% （P=0.04）. CONCLUSION： HSP70 expression in stage Ⅱ-Ⅲ NPC tissues is positively correlated to EBV IgA/VCA titer. The prognosis of HSP70-positive NPC patients is poor.