结直肠癌组织中PDGFRα和PDGFRβ的表达及意义

Expression and Clinical Significance of PDGFRα and PDGFRβ in Colorectal Cancer

背景与目的:血小板衍生生长因子受体α(platelet-derived growth factor receptor-alpha,PDGFRα)和血小板衍生生长因子受体β(platelet-derived growth factor receptor-beta,PDGFRβ)在肿瘤浸润、转移过程中起重要作用,但其与结直肠癌的关系研究甚少。本研究检测结直肠癌组织中PDGFRα和PDGFRβ的表达,并对其与结直肠癌患者临床病理特征和预后的关系进行探讨。方法:采用免疫组化SABC法检测122例结直肠癌组织与17例正常结直肠组织中PDGFRα和PDGFRβ的表达,并分析二者表达与结直肠癌患者临床病理特征及预后的关系。结果:结直肠癌组织中PDGFRα高表达率为68.8%,PDGFRβ高表达率为65.6%,正常结直肠组织中均无高表达。结直肠癌组织中PDGFRα与PDGFRβ表达呈正相关(r=0.416,P<0.001);PDGFRα的表达与原发灶分期、淋巴结转移、远处转移及Dukes’分期正相关。PDGFRβ高表达率在Dukes’C和Dukes'D中较Dukes'A和Dukes'B为高(73.8%vs.56.1%,P=0.040)。PDGFRα高表达患者3年总生存率及3年无进展生存率显著低于低表达者(61.5%vs.72.1%,43.2%vs.72.8%,P<0.05);PDGFRα阳性表达者3年无进展生存率也显著低于阴性表达者(47.3%vs.72.1%,P<0.05)。多因素分析显示,PDGFRα和PDGFRβ的表达不是独立的预后因素。结论:结直肠癌组织中存在PDGFRα和PDGFRβ高表达,PDGFRα和PDGFRβ表达与结直肠癌进展相关。PDGFRα和PDGFRβ的表达不是独立的预后因素。

BACKGROUND ＆ OBJECTIVE： Platelet-derived growth factor receptor-alpha （PDGFRα） and -beta （PDGFRβ） play important roles in the invasion and metastasis of solid tumors. However, their correlations to colorectal cancer have seldom been reported. This study was to detect the expression of PDGFRα and PDGFRβ in colorectal cancer, and investigate their clinical significance. METHODS： The expression of PDGFRa and PDGFRβ in 122 specimens of colorectal cancer and 17 specimens of normal colorectal tissues was detected by SABC immunohistochemistry. The correlations of their expression to the clinicopathologic characteristics and prognosis of the colorectal cancer patients were analyzed. RESULTS： The high expression rates of PDGFRα and PDGFRβ were 68.8% and 65.6% in colorectal cancer, but no high expression was found in normal colorectal tissues. PDGFRα expression was positively correlated to PDGFRβ expression in colorectal cancer （r=0.416, P〈0.001）. The expression of PDGFRα was positively correlated to the stage of primary lesion, regional lymph node metastasis, distant metastasis and Dukes＇ stage. The high expression rate of PDGFRβ was significantly higher in Dukes＇ C and D tumors than in Dukes＇ A and B tumors （73.8% vs. 56.1%, P=0.040）. The 3-year overall and progression-free survival rates were significantly lower in the patients with high PDGFRa expression than in those with low PDGFRα expression （61.5% vs. 72.1%, 43.2% vs. 72.8%, P〈0.05）; the 3-year progression-free survival rate was significantly lower in PDGFRα-positive patients than in PDGFRα-negative patients （47.3% vs. 72.1%, P〈0.05）. On multivariate analysis, both PDGFRα and PDGFRβ were not independent prognostic factors of colorectal cancer. CONCLUSIONS： PDGFRα and PDGFRβ are overexpressed in colorectal cancer, and are related with the progression of colorectal cancer. Both PDGFRa and PDGFRβ are not independent prognostic factors of colorectal cancer.