摘要
目的研究血红素氧合酶-1(HO-1)/一氧化碳(CO)系统在肺纤维化大鼠中的表达及药物干预影响,为肺纤维化的诊治提供一种新方法。方法应用健康Wistar大鼠60只,随机分为4组,分别为对照组、肺纤维化组(纤维化组)、肺纤维化加HO-1特异激动剂组(加强组)、肺维化加HO-1特异抑制剂组(抑制组)。检测各个模型组中大鼠肺组织与血清中HO-1/CO体系表达情况,分析HO-1/CO体系在大鼠肺纤维化中的作用,以及药物对肺纤维化程度的影响。结果在对照组中,HO-1与COHb在大鼠肺组织和血清中低表达,且两者差异无统计学意义(P>0·05),在单纯组、加强组、抑制组中,与对照组相比,HO-1与COHb在大鼠肺组织和血清中均高表达,以加强组为重,抑制组最低,两者差异具有统计学意义(P<0·05)。结论HO-1/CO系统参与了大鼠肺纤维化形成过程,通过对其的干预,可以改变大鼠肺纤维化的程度,为肺纤维化的诊断与治疗的提供新途径。
Objective To investigate the expression and intervention of medicion of heme oxygenase-1/CO in idiopathic pulmonary fibrosis.To offer new method for early diagmosis and therapy of idiopathic pulmonary fibrosis.Methods 60 healty wistar rats was divided into four groups randormly.Discern for：nornal control group、idiopathic pulmonary fibrosis group、the group of specific stimulator of HO-1,the group of specific inhibitorof HO-1.Measured the levels of HO-1 activity and COHb in blood and lung tissues of each group,to analyze effection of HO-1/CO,and influence of medinces in idiopathic pulmonary fibrosis.Results HO-1and COHb was expressd very lowly in blood and lung tissues,and there was not association between HO-1 expression and COHb expression（P〉0.05）.In idiopathic pulmonary fibrosis group,group of specific stimulator and the group of specific inhibitor,HO-1 expression and COHb expression in blood and lung tissues was higher than the goup of control, and there was significant association between HO-1 expression and COHb expression（P〈0.05）.Conclusion The system of HO-1/CO participate in the process of idiopathic pulmonary fibrosis in rats.The extent of idiopathic pulmonary fibrosis can be changed by intervention of medicion of heme oxygenase-1/CO.Therefore,it maybe a new method of pulmonary fibrosis diagnosis and therapy.
出处
《中国实用医药》
2008年第15期39-41,共3页
China Practical Medicine
